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Recombinant Drosophila melanogaster Transformer-2 sex-determining protein (tra2)

  • 中文名稱:
    黑腹果蠅tra2重組蛋白
  • 貨號:
    CSB-YP323071DLU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    黑腹果蠅tra2重組蛋白
  • 貨號:
    CSB-EP323071DLU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    黑腹果蠅tra2重組蛋白
  • 貨號:
    CSB-EP323071DLU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    黑腹果蠅tra2重組蛋白
  • 貨號:
    CSB-BP323071DLU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    黑腹果蠅tra2重組蛋白
  • 貨號:
    CSB-MP323071DLU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    tra2
  • Uniprot No.:
  • 別名:
    tra2; tra-2; CG10128; Transformer-2 sex-determining protein
  • 種屬:
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-264
  • 氨基酸序列
    MDREPLSSGR LHCSARYKHK RSASSSSAGT TSSGHKDRRS DYDYCGSRRH QRSSSRRRSR SRSSSESPPP EPRHRSGRSS RDRERMHKSR EHPQASRCIG VFGLNTNTSQ HKVRELFNKY GPIERIQMVI DAQTQRSRGF CFIYFEKLSD ARAAKDSCSG IEVDGRRIRV DFSITQRAHT PTPGVYLGRQ PRGKAPRSFS PRRGRRVYHD RSASPYDNYR DRYDYRNDRY DRNLRRSPSR NRYTRNRSYS RSRSPQLRRT SSRY
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Required for female sex determination in somatic cells and for spermatogenesis in male germ cells. Positive regulator of female-specific splicing and/or polyadenylation of doublesex (dsx) pre-mRNA. Splicing requires an enhancer complex, dsxRE (dsx repeat element: which contains six copies of a 13-nucleotide repeat and a purine-rich enhancer (PRE)). DsxRE is formed through cooperative interactions between tra, tra2 and the sr proteins, and these interactions require both the repeat sequences and PRE. PRE is required for specific binding of tra2 to the dsxRE. Protein-RNA and protein-protein interactions are involved in tra-2 dependent activation and repression of alternative splicing. Together with tra-2, plays a role in switching fru splicing from the male-specific pattern to the female-specific pattern through activation of the female-specific fru 5'-splice site.
  • 基因功能參考文獻:
    1. Together, these results suggest a link between mRNA splicing, sex determination and lipid metabolism and may provide insight into the mechanisms underlying tissue-specific splicing and nutrient storage. PMID: 29203241
    2. results indicate the Hfp/Puf68 protein is required for the negative regulation of splicing by Tra2 PMID: 23880637
    3. Anastrepha Tra2 protein supplies tra-2 function in Drosophila PMID: 22252495
    4. Tra2 represses M1 splicing by interacting with multiple sequences in the pre-mRNA and interfering with enhancer function PMID: 12861004
    5. Here we show that that widely used tra2 mutant alleles have residual activity and are not suitable for evaluating its effect on viability. PMID: 14595843
    6. Rbp1 and Tra2 bind to the ISS independently through distinct sequences suggesting that functionally synergistic interactions of these SR factors can cause either splicing activation or repression. PMID: 17101798

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  • 蛋白家族:
    Splicing factor SR family
  • 組織特異性:
    Isoform Tmaj and isoform Tmin are expressed in males and females. Isoform msTmaj and isoform msTmin are present only in male germ cells.
  • 數據庫鏈接:

    KEGG: dme:Dmel_CG10128

    STRING: 7227.FBpp0088562

    UniGene: Dm.4216



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