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  4. Recombinant Drosophila melanogaster Serine/threonine-protein kinase fused (fu), partial

Recombinant Drosophila melanogaster Serine/threonine-protein kinase fused (fu), partial

  • 中文名稱：
    黑腹果蠅fu重組蛋白
  • 貨號：
    CSB-YP338537DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅fu重組蛋白
  • 貨號：
    CSB-EP338537DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅fu重組蛋白
  • 貨號：
    CSB-EP338537DLU-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅fu重組蛋白
  • 貨號：
    CSB-BP338537DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅fu重組蛋白
  • 貨號：
    CSB-MP338537DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    fu
  • Uniprot No.：
  • 別名：
    fu; CG6551Serine/threonine-protein kinase fused; EC 2.7.11.1
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Probable serine/threonine-protein kinase; maternally required for correct patterning in the posterior part of each embryonic metamere. May be involved in control of cell division during metamorphosis and ovarian development. May interact with costal-2.
  • 基因功能參考文獻：
    1. Data show that normal Ci-155 activation by Hh requires Cos2 binding to Fu, supporting the hypothesis that Cos2 mediates the apposition of Fu molecules suitable for cross-phosphorylation and consequent full activation of Fu kinase. PMID: 25633354
    2. Transduction of the Hedgehog signal through the dimerization of Fused and the nuclear translocation of Cubitus interruptus. PMID: 21844892
    3. Hh-induced Smo conformational change facilitates the assembly of active Smo-Cos2-Fu signaling complexes that promote Fu kinase domain dimerization, phosphorylation and activation; Fu regulates both the activator and repressor forms of Ci PMID: 21852395
    4. Study shows that Fused (Fu), a serine/threonine kinase that regulates Hedgehog, functions in concert with the E3 ligase Smurf to regulate ubiquitination and proteolysis of the BMP receptor Thickveins in germline stem cells daughter cystoblasts. PMID: 21145463
    5. model in which Hedgehog signals at least twice in germarial somatic cells: through a fused-independent pathway to control somatic stem cell proliferation; and through a classical fused-dependent pathway to regulate prefollicular cell differentiation PMID: 12163413
    6. Data show that the Costal2-Fused-Cubitus interruptus (Cos2-Fu-Ci) protein complex is associated with Smoothened (Smo) in membrane fractions, and that Cos2 binding on Smo is necessary for the Hedgehog-dependent dissociation of Ci from this complex. PMID: 14523402
    7. Hedgehog signaling requires an intramolecular association between two domains of the protein kinase Fused PMID: 15542847
    8. positive role for Fused in the transduction of somatic Hedgehog signaling instructing ovariole morphogenesis PMID: 16145667
    9. we provide evidence that fused regulates these cystocyte divisions cell autonomously, while the tumorous phenotype probably reflects both a somatic and germline requirement for fused for cyst and follicle development. PMID: 16516445
    10. Findings reveal a novel positive-feedback loop in Hedgehog transduction and support a model in which Fused and SMO enhance each other's activities & sustain high levels of signaling and render the pathway robust to Patched level fluctuations. PMID: 17658259
    11. Data suggest that, after activation of Smoothened, the Fused kinase induces a conformational change in Costal2 that allows the disassembly of the Smo-Fu-Cos2-Cubitus interruptus complex and consequent activation of Hedgehog target genes. PMID: 17881487
    12. This protein is a component of the Ci signaling complex that regulates Ci nuclear import and cleavage. PMID: 11476581

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  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 組織特異性：
    Expressed in all imaginal disks, higher level in wing disk.
  • 數據庫鏈接：


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