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  4. Recombinant Drosophila melanogaster Semaphorin-1A (Sema-1a), partial

Recombinant Drosophila melanogaster Semaphorin-1A (Sema-1a), partial

  • 中文名稱：
    黑腹果蠅Sema1a重組蛋白
  • 貨號：
    CSB-YP631541DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Sema1a重組蛋白
  • 貨號：
    CSB-EP631541DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Sema1a重組蛋白
  • 貨號：
    CSB-EP631541DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Sema1a重組蛋白
  • 貨號：
    CSB-BP631541DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Sema1a重組蛋白
  • 貨號：
    CSB-MP631541DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Sema1a
  • Uniprot No.：
  • 別名：
    Sema1a; Dsema-I; Sema-1a; CG18405; Semaphorin-1A; Semaphorin-I; Sema I
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in growth cone guidance through its role in axonal repulsion.
  • 基因功能參考文獻：
    1. The PDZ domain binding motif found in the most C-terminal region of the Sema-1a ICD is necessary for interaction with Vari, but not Cher, indicative of distinct binding modalities. Pbl/Sema-1a-mediated repulsive guidance is potentiated by both vari and cher Genetic analyses further suggest that scaffolding functions of Vari and Cher play an important role in Pbl-mediated Sema-1a reverse signaling. PMID: 28894005
    2. The transmembrane proteins semaphorin-1a (Sema-1a) and plexin A function together to regulate ellipsoid body laminated ring (R) neuron axon lamination. PMID: 28632130
    3. Sema-1a reverse signaling can elicit distinct axonal responses depending on differential engagement of distinct ligands and signaling effectors. PMID: 28052247
    4. Sema1a and Dscam1 might play essential roles in the Drosophila DA1 ventral olfactory projection neurons morphogenesis. PMID: 27163287
    5. these results demonstrate that Sema-1a repulsion keeps dendrites of different types of PNs away from each other, enabling the same types of PN dendrites to be sorted into destined AL glomeruli and permitting for functional assembly of olfactory circuitry. PMID: 28448523
    6. The authors show that PlexA/Sema1a mediates motoneuron axon branching in ways that differ in the proximal femur and distal tibia, based on motoneuronal birth order. PMID: 26926907
    7. A novel mechanism underlying upregulation of axon-axon attraction by Semaphorin-1a (Sema1a) reverse signaling in the developing Drosophila visual system, is reported. PMID: 25049408
    8. This study demonistrated that the transmembrane guidance cue Sema-1a regulates axon-axon interactions, providing insight into links between guidance cue recognition and subsequent intracellular signaling events. PMID: 23177958
    9. Sema1a and netrin A can specifically promote growth through induction of key cellular growth regulators. PMID: 20162636
    10. By co-expressing shibire(ts) and semaphorin 1a, data show that semaphorin 1a was one of the proteins being regulated by endocytosis and its removal was a necessary part of the program for synaptogenesis. PMID: 12835384
    11. Nervy couples cAMP-PKA signaling to Plexin A to regulate Semaphorin 1a-mediated axonal repulsion PMID: 14976319
    12. The function of Sema1a in R-cell axon guidance absolutely requires its cytoplasmic domain and functions as a receptor in regulating R-cell axon guidance in the Drosophila visual system. PMID: 16611816
    13. Thus, graded expression of Sema-1a contributes to connection specificity from ORNs to PNs and then to higher brain centers, ensuring proper representation of olfactory information in the brain. PMID: 17254975

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Semaphorin family
  • 組織特異性：
    Expressed by subsets of neurons and muscles.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG18405

    STRING: 7227.FBpp0302962

    UniGene: Dm.13264



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