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  4. Recombinant Drosophila melanogaster Ribonuclease Z, mitochondrial (JhI-1), partial

Recombinant Drosophila melanogaster Ribonuclease Z, mitochondrial (JhI-1), partial

  • 中文名稱：
    黑腹果蠅JhI-1重組蛋白
  • 貨號：
    CSB-YP847585DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅JhI-1重組蛋白
  • 貨號：
    CSB-EP847585DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅JhI-1重組蛋白
  • 貨號：
    CSB-EP847585DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅JhI-1重組蛋白
  • 貨號：
    CSB-BP847585DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅JhI-1重組蛋白
  • 貨號：
    CSB-MP847585DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    JhI-1
  • Uniprot No.：
  • 別名：
    RNaseZ; JhI-1; CG3298; Ribonuclease Z; mitochondrial; RNase Z; EC 3.1.26.11; Juvenile hormone-inducible protein 1; dRNAse Z; tRNA 3 endonuclease; DmeZ; tRNase Z
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity of nuclear and mitochondrial pre-tRNA. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA. May participate in tRNA processing in the developing embryo.
  • 基因功能參考文獻：
    1. The linker region was explored by Ala-scanning through two conserved regions of D. melanogaster tRNase Z: NdomTprox, located at the carboxy end of the amino domain proximal to the linker, and Tflex, a flexible site in the linker. Periodic substitutions in a hydrophobic patch (F329 and L332) at the carboxy end of NdomTprox show 2,700 and 670-fold impairment relative to wild type PMID: 29045449
    2. The study implicates Drosophila RNase Z(L) in a novel retrograde signaling pathway initiated by the damage in mitochondria and manifested in a cell cycle delay before the mitotic entry. PMID: 26553808
    3. RNase Z(L) activity is essential for all phases of fly development that involve cell division, including growth of adult tissue progenitors during larval and metamorphic stages, and gametogenesis in adults. PMID: 23867108
    4. The study shows that dRNaseZ is necessary for 3'-end processing of nuclear & mitochondrial tRNAs. In mitotically growing imaginal discs it is required for cell proliferation and/or viability. In endoreplicating salivary glands, it controls organ size. PMID: 21146608
    5. conserved, functionally important residues within the His cluster of tRNase Z are thus involved in catalysis, but substrate recognition and binding functions must reside elsewhere in the protein. PMID: 15935379
    6. Residues in two homology blocks on the amino side of the tRNase Z His domain contribute unexpectedly to pre-tRNA 3' end processing PMID: 16618969

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  • 亞細胞定位：
    Nucleus. Mitochondrion.
  • 蛋白家族：
    RNase Z family
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG3298

    STRING: 7227.FBpp0087449

    UniGene: Dm.2795



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