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  4. Recombinant Drosophila melanogaster Regulator of G-protein signaling loco (loco), partial

Recombinant Drosophila melanogaster Regulator of G-protein signaling loco (loco), partial

  • 中文名稱：
    黑腹果蠅loco重組蛋白
  • 貨號：
    CSB-YP896074DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅loco重組蛋白
  • 貨號：
    CSB-EP896074DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅loco重組蛋白
  • 貨號：
    CSB-EP896074DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅loco重組蛋白
  • 貨號：
    CSB-BP896074DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅loco重組蛋白
  • 貨號：
    CSB-MP896074DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    loco
  • Uniprot No.：
  • 別名：
    loco; CG5248; Regulator of G-protein signaling loco; RGS; Locomotion defects protein; Loco
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI and GAP activities on G(i) alpha subunit Galphai. Confers GAP activity on G(o) alpha subunit Galphao and G(i) alpha subunit Galphai. Involved in the dorsal-ventral axis formation of the egg. Acts as a G-protein signaling for glial cell differentiation during embryogenesis; Galphai, Galphao and the G-protein coupled receptor, moody, are required in the surface glia to achieve effective insulation of the nerve cord. May be essential for nurse cell dumping during oogenesis. Required in neuroblast asymmetrical cell division. Plays a role in stress resistance and life span control.
  • 基因功能參考文獻：
    1. RGS proteins, such as Loco, with with GTPase-activating protein (GAP) activity affect both stress resistance and longevity in several species. PMID: 21255223
    2. Loco was identified and a functional analysis made. PMID: 15313576
    3. specific isoforms of loco are required for the differentiation of the male gonad and genital disc PMID: 18387173
    4. Rap/Fzr targets Loco for ubiquitination, thereby regulating glial differentiation in the developing nervous system. PMID: 18430931
  • 亞細胞定位：
    Cytoplasm. Cell membrane. Apical cell membrane. Note=Recruited from the cytosol to the plasma membrane by the G(i) alpha subunit. Colocalizes with moody at the plasma membrane. Localizes as a crescent to the apical cortex in interphase and forms an apical crescent and segregates into the apical daughter cell at telophase. Colocalizes with Galphai and raps/pins at the apical cortex throughout mitosis.
  • 組織特異性：
    Expressed in surface and longitudinal glial cells, gut and heart (at protein level).
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG5248

    STRING: 7227.FBpp0083666

    UniGene: Dm.3231



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