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  4. Recombinant Drosophila melanogaster Ras-related protein Ral-a (Rala)

Recombinant Drosophila melanogaster Ras-related protein Ral-a (Rala)

  • 中文名稱：
    黑腹果蠅Rala重組蛋白
  • 貨號：
    CSB-YP019296DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Rala重組蛋白
  • 貨號：
    CSB-EP019296DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Rala重組蛋白
  • 貨號：
    CSB-EP019296DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Rala重組蛋白
  • 貨號：
    CSB-BP019296DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Rala重組蛋白
  • 貨號：
    CSB-MP019296DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Rala; CG2849; Ras-related protein Ral-a
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-198
  • 氨基酸序列
    MSKKPTAGPA LHKVIMVGSG GVGKSALTLQ FMYDEFVEDY EPTKADSYRK KVVLDGEEVQ IDILDTAGQE DYAAIRDNYF RSGEGFLCVF SITDDESFQA TQEFREQILR VKNDESIPFL LVGNKCDLND KRKVPLSECQ LRAQQWAVPY VETSAKTREN VDKVFFDLMR EIRSRKTEDS KATSGRAKDR CKKRRLKC
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 基因功能參考文獻：
    1. data indicate that Ral and the exocyst regulate autophagy in a context-dependent manner, and that in dying salivary glands, Ral mediates autophagy, at least in part, by regulation of Notch. PMID: 26598552
    2. These studies identify a pathway, which stretches from Rab8 to RalA and the exocyst complex, that mediates rapid furrow formation in early Drosophila embryos. PMID: 26092850
    3. Constitutively active Ral expressed in muscle causes the exocyst to be concentrated in the region surrounding synaptic boutons and consequently enlarges the membrane folds of the postsynaptic plasma membrane. PMID: 23812009
    4. the Rap1-Rgl-Ral signaling network is a novel mechanism that cooperates with other intrinsic polarity cues to modulate asymmetric neuroblast division PMID: 22084305
    5. prevention of ligand-independent Notch activation is not simply a constitutive process, but is a target for regulation by Ral during cell fate specification and pattern formation. PMID: 21350007
    6. Abnormalities of the Wnt5/5a pathway (located in the apical ectodermal ridge as well as in the mesoderm) will affect chondrogenesis of the developing limb and experimental Wnt5a(-/-) limbs have terminal adactyly. PMID: 20709709
    7. the Ral-centered protein network appears to be extremely conserved in human and flies PMID: 12529414
    8. The identification and characterization of a DBL-like guanine nucleotide exchange factor (GEF) in Drosophila, called GEFmeso, as a novel binding target of the Ras-like GTPase Ral. PMID: 16581976
    9. RalA is required in organizing centres to control proper patterning and migration in vivo. PMID: 18552769

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  • 亞細胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Cleavage furrow. Midbody, Midbody ring.
  • 蛋白家族：
    Small GTPase superfamily, Ras family
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG2849

    STRING: 7227.FBpp0070558

    UniGene: Dm.1689



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