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  4. Recombinant Drosophila melanogaster Ras-like GTP-binding protein Rho1 (Rho1)

Recombinant Drosophila melanogaster Ras-like GTP-binding protein Rho1 (Rho1)

  • 中文名稱：
    黑腹果蠅Rho1重組蛋白
  • 貨號：
    CSB-YP343711DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Rho1重組蛋白
  • 貨號：
    CSB-EP343711DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Rho1重組蛋白
  • 貨號：
    CSB-EP343711DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Rho1重組蛋白
  • 貨號：
    CSB-BP343711DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Rho1重組蛋白
  • 貨號：
    CSB-MP343711DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Rho1
  • Uniprot No.：
  • 別名：
    Rho1; CG8416; Ras-like GTP-binding protein Rho1
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-189
  • 氨基酸序列
    MTTIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRNDPNTIRD LAKMKQEPVK PQEGRAMAEK INAFAYLECS AKSKEGVRDV FETATRAALQ VKKRKKTRC
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Has a role in regulating actin cytoskeletal organization: required during early development for proper execution of morphogenetic movements of individual cells and groups of cells important for the formation of the embryonic body plan. Plays a role in regulating dorsal closure during embryogenesis. During axis elongation, required for Rho-kinase Rok planar polarity and adherens junction localization as well as for generating a planar polarized distribution of the actin-binding protein Shrm. During embryogenesis, acts upstream of wash to regulate the developmental migration of tail hemocytes anteriorly along the ventral midline. May have a role in eye development.
  • 基因功能參考文獻：
    1. Study identified circadian rhythms in Rho1 GTPase activity in s-LNv axons that are regulated by rhythmic transcription of Pura, a Rho1 GEF. Pura activates Rho1 to retract s-LNv axons, decrease active zone numbers and reduce s-LNvs' influence on the clock network. Thus, study links transcription, plasticity, network hierarchy and behavior. PMID: 26234154
    2. As tension in the epithelial layer increases, Rho kinase signaling activates myosin assembly and contraction in the developing Drosophila egg chamber. PMID: 24943847
    3. This work identifies spatially distinct functions for Rho1 in the regulation of DE-cadherin-containing vesicular trafficking during adherens junctions remodeling in live epithelia. PMID: 25079692
    4. impaired Hippo signaling induces JNK activation through Rho1. PMID: 25583514
    5. Galpha73B is a downstream effector of JAK/STAT signaling and a regulator of Rho1 in Drosophila hematopoiesis. PMID: 24163435
    6. The RhoA fails to form a compact ring in late cytokinesis after Sti depletion, and this function requires Sti kinase activity. PMID: 22084308
    7. removal of a single copy of rho1 can suppress the pak phenotype PMID: 21098722
    8. Results suggest that Crossveinless-c contributes to sprouting and subsequent growth of the anterior-posterior-oriented branches through negative regulation of Rho1. PMID: 20384791
    9. Continuous autophagy was required for integrin-mediated hemocyte spreading and Rho1-induced cell protrusions. PMID: 20498061
    10. disruption of Rho1 suppresses apical constriction and invagination in Adenomatous polyposis coli null cells PMID: 20102708
    11. Results suggest that Rho1 functions at the cell cortex to regulate JNK activity and implicate Rho1 and moesin in epithelial cell survival. PMID: 20404112
    12. analysis of second-site noncomplementation screen for modifiers of Rho1 signaling during imaginal disc morphogenesis in Drosophila PMID: 19862331
    13. Drosophila RhoA regulates the cytoskeleton and cell-cell adhesion in the developing epidermis. PMID: 12070092
    14. While Rho1 protein is present throughout the cell, it accumulates apically, particularly at sites of cadherin-based adherens junctions. PMID: 12135916
    15. RhoA and Sb-sbd act in a common pathway during leg morphogenesis PMID: 14668391
    16. role for Rho1 in regulating signaling events governing proper patterning during development PMID: 15649467
    17. Taken together, these findings suggest that RhoGEF4 may participate in cytoskeleton-related cellular events by specifically activating RhoA in neuronal morphogenesis. PMID: 17011730
    18. Reducing activity of Rho1 from somatic support cells suppressed the germ cell enclosure defects of the conditional Spitz allele. PMID: 17629483
    19. In parallel to regulation of crb RNA and protein, Rho1 activity also signals through Rho-kinase (Rok) to induce apical constriction and cell shape change during invagination of salivary gland. PMID: 18585373
    20. Results demonstrate that Rho1 is required to maintain adherens junction integrity independent of its role in sustaining apical cell tension. PMID: 19506041
    21. in addition to its interaction with Mwh Rho1 has functions in wing planar polarity that are parallel to and upstream of fz. PMID: 19576201

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  • 亞細胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Apical cell membrane. Lateral cell membrane.
  • 蛋白家族：
    Small GTPase superfamily, Rho family
  • 組織特異性：
    Expressed in hemocytes (at protein level).
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG8416

    STRING: 7227.FBpp0086354

    UniGene: Dm.644



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