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  4. Recombinant Drosophila melanogaster Raf homolog serine/threonine-protein kinase phl (phl), partial

Recombinant Drosophila melanogaster Raf homolog serine/threonine-protein kinase phl (phl), partial

  • 中文名稱：
    黑腹果蠅phl重組蛋白
  • 貨號：
    CSB-YP319284DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅phl重組蛋白
  • 貨號：
    CSB-EP319284DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅phl重組蛋白
  • 貨號：
    CSB-EP319284DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅phl重組蛋白
  • 貨號：
    CSB-BP319284DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅phl重組蛋白
  • 貨號：
    CSB-MP319284DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    phl
  • Uniprot No.：
  • 別名：
    Raf; ph; phl; CG2845; Raf homolog serine/threonine-protein kinase Raf; D-Raf; dRAF-1; EC 2.7.11.1; Protein pole-hole
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Serine/threonine kinase required in the early embryo for the formation of terminal structure. Also required during the proliferation of imaginal cells. May act downstream of Ras85D in the tor signal transduction pathway. During larval development, mediates Ptth/tor signaling leading to the production of ecdysone, a hormone required for the initiation of metamorphosis.
  • 基因功能參考文獻：
    1. Raf-activated intestinal stem cells and their progenies are very sensitive to apoptosis PMID: 26845534
    2. Yorkie (Yki), a transcriptional coactivator in the Hippo pathway that regulates organ size, is required for Raf-induced cardiac hypertrophy in flies PMID: 25650441
    3. move from the oocyte to the follicle cells in process that does not require endocytosis PMID: 25483249
    4. These results suggest that ERK is necessary, but not sufficient, for Raf-mediated cardiac hypertrophy. PMID: 23580199
    5. RNAi-mediated inhibition of MAPK-signaling by knockdown of Ras85D, phl/D-Raf or Dsor1/MEK affects larval development, adult life span and hemolymph phosphate, suggesting that some in vivo effects involve activation of this signaling pathway by phosphate. PMID: 23520455
    6. role in promotion of blood cell activation and differentiation in conjunction with hopscotch jak kinase PMID: 11919715
    7. pTyr 1253 (YE) Neu (ErbB-2) autophosphorylation site signalling requires Raf function. PMID: 12673197
    8. the integration of RTK-induced RAS and Src42 signals by CNK as a two-component input is essential for RAF activation PMID: 15660123
    9. tyrosine 510 regulates Draf activation by affecting the autoinhibitory interaction between the N- and C-terminal fragments of the protein PMID: 18494562
    10. RAF catalytic function is regulated in response to a specific mode of dimerization of its kinase domain, which we term the side-to-side dimer PMID: 19727074

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  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG2845

    UniGene: Dm.39



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