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  4. Recombinant Drosophila melanogaster Putative ATP-dependent RNA helicase me31b (me31B)

Recombinant Drosophila melanogaster Putative ATP-dependent RNA helicase me31b (me31B)

  • 中文名稱：
    黑腹果蠅me31B重組蛋白
  • 貨號(hào)：
    CSB-YP326039DLU
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅me31B重組蛋白
  • 貨號(hào)：
    CSB-EP326039DLU
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅me31B重組蛋白
  • 貨號(hào)：
    CSB-EP326039DLU-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅me31B重組蛋白
  • 貨號(hào)：
    CSB-BP326039DLU
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅me31B重組蛋白
  • 貨號(hào)：
    CSB-MP326039DLU
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    me31B
  • Uniprot No.：
  • 別名：
    me31B; CG4916ATP-dependent RNA helicase me31b; EC 3.6.4.13; Maternal expression at 31B
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-459
  • 氨基酸序列
    MMTEKLNSGH TNLTSKGIIN DLQIAGNTSD DMGWKSKLKL PPKDNRFKTT DVTDTRGNEF EEFCLKRELL MGIFEKGWER PSPIQEAAIP IALSGKDVLA RAKNGTGKTG AYCIPVLEQI DPTKDYIQAL VMVPTRELAL QTSQICIELA KHLDIRVMVT TGGTILKDDI LRIYQKVQLI IATPGRILDL MDKKVADMSH CRILVLDEAD KLLSLDFQGM LDHVILKLPK DPQILLFSAT FPLTVKNFME KHLREPYEIN LMEELTLKGV TQYYAFVQER QKVHCLNTLF SKLQINQSII FCNSTQRVEL LAKKITELGY CCYYIHAKMA QAHRNRVFHD FRQGLCRNLV CSDLFTRGID VQAVNVVINF DFPRMAETYL HRIGRSGRFG HLGIAINLIT YEDRFDLHRI EKELGTEIKP IPKVIDPALY VANVGASVGD TCNNSDLNNS ANEEGNVSK
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    ATP-dependent RNA helicase which is a core component of a variety of ribonucleoprotein complexes (RNPs) that play critical roles in translational repression and mRNA decapping during embryogenesis, oogenesis, neurogenesis and neurotransmission. Recruits core components and translational repressors to some RNP complexes, and mediates RNP aggregation into processing granules such as P-bodies. As part of a RNP complex containing tral, eIF4E1, cup, and pAbp, involved in RNP-mediated translational repression of maternal mRNAs during oogenesis and embryogenesis. As part of a RNP complex containing tral and the RNA localization factors exu and yps, mediates translational silencing of mRNAs such as osk/oskar and bcd/bicoid during their transport to the oocyte in order to prevent their translation until they reach their positional destinations. In neurons and possibly imaginal disks, involved in miRNA-mediated translational repression, possibly in association with components of the piRNA transposon silencing pathway. Involved in RNA localization and protein trafficking in the oocyte. As part of an ER-associated RNP containing tral, cup and yps, required for tral-dependent ER exit site formation and consequently efficient trafficking of proteins such as grk and yl through the secretory pathway. Component of neuron RNPs that mediate transport and translation of neuronal RNAs, including translation repression of synaptic transcripts in preparation for their dendritic targeting. As part of the Atx2-Not1 repressor complex promotes Not1-dependent post-transcriptional gene silencing in adult circadian pacemaker neurons in order to sustain high-amplitude circadian rhythms and Pdf cycling in a per-independent manner. Promotes the interaction between Atx2 and Not1 within the Atx2-Not1 RNP complex. Recruited to the 4EHP-GYF2 complex by Gyf, where it plays a role in 4EHP-GYF2 mediated translational repression and mRNA decay.
  • 基因功能參考文獻(xiàn)：
    1. The authors show that the RNA-binding protein, ME31B, binds to and represses the expression of thousands of maternal mRNAs during the Drosophila maternal-to-zygotic transition. PMID: 28875934
    2. study supports the role of Me31B in RNA regulation and suggests its novel roles in germ granule assembly and function. PMID: 28945271
    3. Me31B role in the translational repression of the Drosophila Nanos mRNA PMID: 28701521
    4. LSM12 and ME31B/DDX6 define distinct modes of posttranscriptional regulation by ATAXIN-2 protein complex in drosophila circadian pacemaker neurons. PMID: 28388438
    5. PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition. PMID: 21447556
    6. Tral and EDC3 specifically interact with the decapping activator DCP1 and the DEAD-box helicase Me31B, in Drosophila cells. PMID: 18765641
    7. Structural basis for the mutually exclusive anchoring of P body components EDC3 and Tral to the DEAD box protein DDX6/Me31B. PMID: 19285948

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  • 亞細(xì)胞定位：
    Cytoplasm. Cytoplasm, Cytoplasmic ribonucleoprotein granule. Cytoplasm, P-body. Endoplasmic reticulum. Cell projection, dendrite.
  • 蛋白家族：
    DEAD box helicase family, DDX6/DHH1 subfamily
  • 組織特異性：
    Ubiquitously expressed throughout the brain (at protein level). Expressed in the olfactory system including the antennal lobes, projection neurons, local interneurons, mushroom-body Kenyon cells and glial cells (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: dme:Dmel_CG4916

    STRING: 7227.FBpp0079565

    UniGene: Dm.2770



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