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  4. Recombinant Drosophila melanogaster Protein vestigial (vg)

Recombinant Drosophila melanogaster Protein vestigial (vg)

  • 中文名稱：
    黑腹果蠅vg重組蛋白
  • 貨號(hào)：
    CSB-YP638869DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅vg重組蛋白
  • 貨號(hào)：
    CSB-EP638869DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅vg重組蛋白
  • 貨號(hào)：
    CSB-EP638869DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅vg重組蛋白
  • 貨號(hào)：
    CSB-BP638869DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅vg重組蛋白
  • 貨號(hào)：
    CSB-MP638869DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    vg
  • Uniprot No.：
  • 別名：
    vg; CG3830Protein vestigial
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-453
  • 氨基酸序列
    MAVSCPEVMY GAYYPYLYGR AGTSRSFYQY ERFNQDLYSS SGVNLAASSS ASGSSHSPCS PILPPSVSAN AAAAVAAAAH NSAAAAVAVA ANQASSSGGI GGGGLGGLGG LGGGPASGLL GSNVVPGSSS VGSVGLGMSP VLSGAAGHSL HSSHRTHAHS LAHAHTHPHS HTHTHTHQTK EEDLIVPRSE AEARLVGSQQ HQHHNESSCS SGPDSPRHAH SHSHPLHGGG GATGGPSSAG GTGSGGGDGG GTGAIPKNLP ALETPMGSGG GGLAGSGQGQ AQYLSASCVV FTNYSGDTAS QVDEHFSRAL NYNNKDSKES SSPMSNRNFP PSFWNSNYVH PIPAPTHHQV SDLYGTATDT GYATDPWVPH AAHYGSYAHA AHAHAAHAHA YHHNMAQYGS LLRLPQQYAS HGSRLHHDQQ TAHALEYSSY PTMAGLEAQV AQVQESSKDL YWF
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in determining which thoracic imaginal disk cells will form wings and halteres, perhaps by interacting with other nuclear regulatory proteins. When in combination with scalloped (sd), it acts as a transcriptional activation complex that regulates gene expression in the wing. Binding to sd switches the DNA target selectivity of sd. Required and sufficient for cell proliferation at the dorsal/ventral (D/V) boundary of the wing imaginal disk. Also required for cell proliferation in the wing imaginal disk, mediated via activation of E2f. By interacting with Dhfr, may control genes involved in DNA replication.
  • 基因功能參考文獻(xiàn)：
    1. High hydrostatic pressure-induced wing mutagenesis in Drosophila melanogaster resulted in 285 differentially expressed genes and two point mutations in the vestigial gene. PMID: 26446369
    2. Data show that genes araucan and caupolican as novel muscle identity genes that confer lateral transverse muscle identity, and the acquisition of this fate requires that Araucan/Caupolican repress other muscle identity genes such as slouch and vestigial. PMID: 21811416
    3. Studies indicate that negative regulation of the Vestigial gradient by Wingless signaling makes vestigial expression precise and robust. PMID: 20932848
    4. Vg is a key factor to induce specific changes in ventral longitudinal muscles 1-4 identity and is required for these cells to be competent to form stable intermuscular attachments with each other. PMID: 20685961
    5. We show that the ectopic expression of vestigial only induces ectopic outgrowths with wing cuticular differentiation and wing blade gene expression patterns in specific developmental and genetic contexts. PMID: 12441303
    6. A negative association between the vg expressiveness and the degree of chromosome polyteny was revealed and proved to be stronger in females than in males. PMID: 12575446
    7. vg appears to be a key regulatory gene of IFM development PMID: 12921740
    8. a critical player for both cell proliferation and cell survival in the presumptive wing margin area PMID: 14526388
    9. Sumoylation by SUMO stimulates Vg function during wing morphogenesis. PMID: 16026969
    10. Differences in vestigial expression between neighboring cells of the wing blade autonomously and nonautonomously affect cell proliferation along the proximo-distal axis. PMID: 16950871
    11. Control of Drosophila wing growth by the vestigial quadrant enhancer. PMID: 17634191
    12. Data show that both Vestigial and Scalloped are required for muscle differentiation, as loss of either leads to a loss of a subset of either cardiac or somatic muscle cells in developing embryos, and also that Sd, Vg, and Dmef2 can interact directly. PMID: 18987343
    13. Data show that vestigial is controlled by the Notch anti-myogenic signaling pathway in myoblasts and is regulated by a novel 822 bp enhancer during indirect flight muscle differentiation. PMID: 19500564

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  • 亞細(xì)胞定位：
    Nucleus.
  • 組織特異性：
    Expressed in the developing wing primordia initially along the D/V wing boundary, and by the late third larval instar, maximal expression is seen in cells at the D/V wing disk boundary. Less expression is seen in cells located farther from this boundary.
  • 數(shù)據(jù)庫(kù)鏈接：


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