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  4. Recombinant Drosophila melanogaster Protein twist (twi)

Recombinant Drosophila melanogaster Protein twist (twi)

  • 中文名稱：
    黑腹果蠅twi重組蛋白
  • 貨號：
    CSB-YP318774DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅twi重組蛋白
  • 貨號：
    CSB-EP318774DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅twi重組蛋白
  • 貨號：
    CSB-EP318774DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅twi重組蛋白
  • 貨號：
    CSB-BP318774DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅twi重組蛋白
  • 貨號：
    CSB-MP318774DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    twi
  • Uniprot No.：
  • 別名：
    twi; CG2956; Protein twist
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-490
  • 氨基酸序列
    MMSARSVSPK VLLDISYKPT LPNIMELQNN VIKLIQVEQQ AYMQSGYQLQ HQQQHLHSHQ HHQQHHQQQH AQYAPLPSEY AAYGITELED TDYNIPSNEV LSTSSNQSAQ STSLELNNNN TSSNTNSSGN NPSGFDGQAS SGSSWNEHGK RARSSGDYDC QTGGSLVMQP EHKKLIHQQQ QQQQQHQQQI YVDYLPTTVD EVASAQSCPG VQSTCTSPQS HFDFPDEELP EHKAQVFLPL YNNQQQQSQQ LQQQQPHQQS HAQMHFQNAY RQSFEGYEPA NSLNGSAYSS SDRDDMEYAR HNALSSVSDL NGGVMSPACL ADDGSAGSLL DGSDAGGKAF RKPRRRLKRK PSKTEETDEF SNQRVMANVR ERQRTQSLND AFKSLQQIIP TLPSDKLSKI QTLKLATRYI DFLCRMLSSS DISLLKALEA QGSPSAYGSA SSLLSAAANG AEADLKCLRK ANGAPIIPPE KLSYLFGVWR MEGDAQHQKA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in the establishment and dorsoventral patterning of germ layers in the embryo.
  • 基因功能參考文獻：
    1. The level of Twi activity determines whether the cellular events of ventral furrow formation, epithelial-to-mesenchymal transition, cell division and mesodermal migration occur. PMID: 24915423
    2. Our studies identify Akirin as a nuclear factor that genetically interacts with the BRM complex and is required for optimal expression of the Twist-dependent Dmef2 enhancer PMID: 22396663
    3. conclude that Twi is an essential Notch co-regulator in myogenic progenitor cells and has the potential to confer specificity on Notch signalling at over 170 genes, showing that a single factor can have a profound effect on the output of the pathway. PMID: 20610485
    4. The role of Twist in the Toll/Dorsal signaling pathway that specifies patterning thresholds of gene expression PMID: 12091311
    5. anterior-gut formation is mechanically induced by the movement of germ-band extension through the induction of Twist expression in stomodeal cells PMID: 12932320
    6. High Twist levels direct cells to the body wall muscle fate, whereas low levels are permissive for gut muscle and fat body fate. PMID: 15128668
    7. twi expression in Drosophila during gastrulation and mesoderm formation was compared to twi expression in the same embryonic development stages in Tribolium castaneum. PMID: 15645317
    8. Twist binds to almost all mesodermal cis-regulatory modules to provide the competence to integrate inputs from more specialized transcription factors. PMID: 17322403
    9. These experiments show that mechanically induced Twist upregulation in stomodeal cells is necessary for subsequent midgut differentiation in Drosophila embryos. PMID: 18804441
    10. Data show that cell fate decisions in the dorsal and lateral Twi- and Tin-positive mesoderm of Drosophila melanogaster depend on the antagonistic action of the transcription factor Lame duck and the zinc finger homeodomain factor Zfh1. PMID: 19028484

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  • 亞細胞定位：
    Nucleus.
  • 組織特異性：
    Expressed in embryonic abdomen; a single cell ventrally, pairs of cells laterally and three cells dorsally in each hemisegment. In the thorax, there are patches of cells associated with the imaginal disks. During larval development, cells proliferate and,
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG2956

    STRING: 7227.FBpp0071864

    UniGene: Dm.5605



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