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Recombinant Drosophila melanogaster Protein shifted (shf)

  • 中文名稱:
    黑腹果蠅shf重組蛋白
  • 貨號:
    CSB-YP893839DLU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    黑腹果蠅shf重組蛋白
  • 貨號:
    CSB-EP893839DLU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    黑腹果蠅shf重組蛋白
  • 貨號:
    CSB-EP893839DLU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    黑腹果蠅shf重組蛋白
  • 貨號:
    CSB-BP893839DLU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    黑腹果蠅shf重組蛋白
  • 貨號:
    CSB-MP893839DLU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    shf
  • Uniprot No.:
  • 別名:
    shf; CG3135; Protein shifted; WIF-1-like protein
  • 種屬:
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度:
    full length protein
  • 表達區域:
    31-456
  • 氨基酸序列
    RQQHHNRNNN NNNRRADSSS SEEGHGNTSD GLDNFADQDA SFVGHGHQPR RGQRKKQQGG GGGGSGGGGG NGGGGGSRHN RNEESGISLW INEQQLKMLT ALYFPQGYSE RLYAIHNSRV TNDLRDTTLY NFLVIPSEVN YVNFTWKSGR RKYFYDFDRL QTMDESILKA PTLSIRKSGR IPQEQKNFSI FLPCTGNSSG TASFNVGLKI QTRHNKPLSG TPIRLNFKKE CAHRGVYDID ASNPTSLTTL QECSLKCGKN GYCNEHHICK CNVGYTGQYC ETAFCFPQCL NGGNCTAPSV CTCPEGYQGT QCEGGICKDK CLNGGKCIQK DKCQCSKGYY GLRCEYSKCV IPCKNEGRCI GNNLCRCPNG LRGDHCEIGR KQRSICKCRN GTCVSHKHCK CHPGFYGRHC NGRKRRHVHR NDDSKF
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Required for normal accumulation and movement of lipid-modified hedgehog (hh) morphogen. May act by stabilizing the interaction between heparan sulfate proteoglycans (HSPGs) and hh, HSPGs being required for diffusion of hh morphogen. Not involved in wingless (wg) morphogen movement, suggesting that it may provide HSPG specificity for Hh.
  • 基因功能參考文獻:
    1. We show that production of this PGC attractant depends upon shf as well as a second hh pathway gene g gamma1. PMID: 24068944
    2. study concludes that the WIF domain is responsible for the divergence between Drosophila Shf and human Wif-1, channeling the recognition either toward Hh or Wg signal, whereas the EGF repeats give the protein the ability to interact with glypicans PMID: 22951645
    3. Shf is the ortholog of the human Wnt inhibitory factor (WIF), a secreted antagonist of the Wingless pathway. Shf is required for Hh stability and for lipid-modified Hh diffusion. PMID: 15691765
    4. Shifted is a secreted protein that acts over a long distance and is required for the normal accumulation of Hh protein and its movement in the wing. PMID: 15691766
  • 亞細胞定位:
    Secreted, extracellular space, extracellular matrix. Note=Colocalizes with HSPG.
  • 組織特異性:
    At the blastoderm stage, it is ubiquitously expressed. As embryogenesis continues, it is expressed in the epidermis and central nervous system, this expression being segmentally modulated. Also highly expressed at the foregut and hindgut throughout embryo
  • 數據庫鏈接:

    KEGG: dme:Dmel_CG3135

    STRING: 7227.FBpp0070939

    UniGene: Dm.58



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