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  4. Recombinant Drosophila melanogaster Protein phyllopod (phyl)

Recombinant Drosophila melanogaster Protein phyllopod (phyl)

  • 中文名稱：
    黑腹果蠅phyl重組蛋白
  • 貨號：
    CSB-YP630652DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅phyl重組蛋白
  • 貨號：
    CSB-EP630652DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅phyl重組蛋白
  • 貨號：
    CSB-EP630652DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅phyl重組蛋白
  • 貨號：
    CSB-BP630652DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅phyl重組蛋白
  • 貨號：
    CSB-MP630652DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    phyl
  • Uniprot No.：
  • 別名：
    phyl; CG10108; Protein phyllopod
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-400
  • 氨基酸序列
    MSTNQQQQAN PSAAVAAPAA SSEYLKRTCL ICGCHTNQTI NIYEPRSGPN IVQLIQAKFK FQPLNEDKFL CFSCNNWLIN WHSLQAVNSN EAESQSQSPS HMGNSVLQQE RTKLRPVAMV RPTVRVQPQS QPQLQPQVPI NPTPAPIVYS KRRASRRSAS VSRMSRVLRQ CCVESLRRSP KKRNQQSVFV CLRPQGQKRS NAICKVECVA PRRKPVERLV KDVAATATPT PVLNTQSTPT YQRFPQPSVD GKVVAMFRRL GTTLSREEPA AYSAESNPAC SKLPQIMSPL KEAPRWTRDL DDDEILLEFD TAISEVLPTA RYQVTHEENK ENQQAQEMEL ELEEEEEVDG RAELEVVQEA EAPLEPQSHH KQGNSHQNSH QASIQLAGLR LPMGLSISLV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Essential adapter component of E3 ubiquitin ligase complexes; involved in R7 photoreceptor cell differentiation, embryonic nervous system, external sensory organ development and specification of particular muscles. E3 ubiquitin ligase complexes mediate ubiquitination and subsequent proteasomal degradation of target proteins. Required for specification of R7 photoreceptor cell fate in the eye by participating in the ubiquitination and subsequent proteasomal degradation of Tramtrack (ttk), a general inhibitor of photoreceptor differentiation. Acts downstream of Notch signaling to specify the fate of the SOP (sensory organ precursor) cells and their progeny, probably via the sina-mediated proteasomal degradation of ttk. Its restricted pattern of expression, upon Notch and Ras signaling pathways, suggests that it acts as a key determinant in E3 complexes to trigger protein proteolysis in appropriate cells.
  • 基因功能參考文獻：
    1. identified Phyllopod (Phyl) as a key regulator that drives enteroendocrine cells generation from intestinal stem cells. PMID: 29276156
    2. Negative feedback regulation of phyl controls the timing of neural precursor division. PMID: 18701547
  • 亞細胞定位：
    Nucleus.
  • 組織特異性：
    In embryos, it is ubiquitously present before cellularization. During stages 9-11, it is expressed in neuroblasts and the SOP cells. From stage 12 onward, it decreases, but remains in a subset of PNS cells at stages 12-14. Weakly expressed in wing imagina
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG10108

    STRING: 7227.FBpp0086645

    UniGene: Dm.5449



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