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  4. Recombinant Drosophila melanogaster Protein pelota (pelo)

Recombinant Drosophila melanogaster Protein pelota (pelo)

  • 中文名稱：
    黑腹果蠅pelo重組蛋白
  • 貨號：
    CSB-YP344149DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅pelo重組蛋白
  • 貨號：
    CSB-EP344149DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅pelo重組蛋白
  • 貨號：
    CSB-EP344149DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅pelo重組蛋白
  • 貨號：
    CSB-BP344149DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅pelo重組蛋白
  • 貨號：
    CSB-MP344149DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    pelo; CG3959; Protein pelota; EC 3.1.-.-
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-395
  • 氨基酸序列
    MKLLGKYVDK GMQGNVTLVP EESEDMWHAY NLIAKGDSVR STTIRKVQNE TATGSSTSSR VRTTLTIAVE SIDFDTQACV LRLKGRNIEE NQYVKMGAYH TLDLELNRKF ELRKPEWDTI ALERIEMACD PTQSADVAAV VMQEGLAHVC LITASMTLVR SKIEVSIPRK RKGSVQQHEK GLAKFYEQVM QSILRHVNFD VVKCVLIASP GFVRDQFYDY MFQQAVKMDY KLLLDNKSKF MLVHASSGFK HSLREILQDP AVLAKMSDTK AAGEVKALEQ FYMMLQCEPA KAFYGKKHVL QAAESQAIET LLISDNLFRC QDVSLRKEYV NLVESIRDAG GEVKIFSSMH ISGEQLAQLT GIAALLRFPM PELEDSDDDD DEDGAAGGVA DSDSD
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway. In the presence of pix and HSB1, is required for 48S complex formation from 80S ribosomes and dissociation of vacant 80S ribosomes. Together with HBS1 and in presence of pix, recognizes stalled ribosomes and promotes dissociation of elongation complexes assembled on non-stop mRNAs; this triggers endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and to degrade damaged mRNAs as part of the No-Go Decay (NGD) pathway. Required prior to the first meiotic division for spindle formation and nuclear envelope breakdown during spermatogenesis. Together with HBS1, promotes spermatid individualization during spermatogenesis. Required for ovarian germ line stem cell self-renewal and oocyte development during oogenesis. Together with HSB1, required for transposon silencing in the ovary and testis. As part of the Pink1-regulated signaling, is recruited to damaged mitochondrial and is required for recruitment of autophagy receptors and induction of mitophagy. Required for normal eye patterning and for mitotic divisions in the ovary.
  • 基因功能參考文獻：
    1. The evolutionarily conserved Pelo (Dom34)-Hbs1 mRNA surveillance complex is required for transposon silencing in the Drosophila germline. PMID: 26124316
    2. data demonstrated that pelo is a host factor that is required for high efficiency translation of viral capsids and targeting pelo could be a strategy for general inhibition of viral infection. PMID: 24722736
    3. Pelo controls GSC self-renewal by repressing a Bam-independent differentiation pathway possibly through regulating translation. PMID: 16280348
  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Eukaryotic release factor 1 family, Pelota subfamily
  • 組織特異性：
    Expressed in ovaries and muscles (at protein level). Expressed throughout all development stages.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG3959

    STRING: 7227.FBpp0079444

    UniGene: Dm.4742



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