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  4. Recombinant Drosophila melanogaster Protein-methionine sulfoxide oxidase Mical (Mical), partial

Recombinant Drosophila melanogaster Protein-methionine sulfoxide oxidase Mical (Mical), partial

  • 中文名稱：
    黑腹果蠅Mical重組蛋白
  • 貨號：
    CSB-YP772974DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Mical重組蛋白
  • 貨號：
    CSB-EP772974DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Mical重組蛋白
  • 貨號：
    CSB-EP772974DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Mical重組蛋白
  • 貨號：
    CSB-BP772974DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Mical重組蛋白
  • 貨號：
    CSB-MP772974DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Mical
  • Uniprot No.：
  • 別名：
    Mical; CG33208[F-actin]-monooxygenase Mical; EC 1.14.13.225; Molecule interacting with CasL protein homolog
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits at 'Met-44' and 'Met-47' through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament disassembly and prevent repolymerization. Plays a key role in semaphorin-plexin repulsive axon guidance and cell morphological changes, probably via its ability to modify and regulate actin.
  • 基因功能參考文獻：
    1. Results identify Mical-like as a novel regulator of synaptic growth and FasII endocytic recycling. PMID: 27770767
    2. Mical oxidizes actin stereospecifically to generate actin Met-44-R-sulfoxide. PMID: 24212093
    3. Mical is both necessary and sufficient for semaphorin-plexin-mediated F-actin reorganization in vivo PMID: 20148037
    4. MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion. PMID: 12110185
    5. Transgenic expression of interfering RNA molecules demonstrates that MICAL is required in muscles for the higher order arrangement of myofilaments. PMID: 17350233

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Mical family
  • 組織特異性：
    Present in neuronal cell bodies, along axons, and in growth cones. Appears in the nervous system at stage 13 and labels motor and CNS projections, and at later embryonic stages, it is present on axons that make up all motor axon pathways: the intersegment
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG33208

    STRING: 7227.FBpp0297564

    UniGene: Dm.32089



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