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  4. Recombinant Drosophila melanogaster Protein male-specific lethal-3 (msl-3), partial

Recombinant Drosophila melanogaster Protein male-specific lethal-3 (msl-3), partial

  • 中文名稱：
    黑腹果蠅msl-3重組蛋白
  • 貨號：
    CSB-YP342300DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅msl-3重組蛋白
  • 貨號：
    CSB-EP342300DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅msl-3重組蛋白
  • 貨號：
    CSB-EP342300DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅msl-3重組蛋白
  • 貨號：
    CSB-BP342300DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅msl-3重組蛋白
  • 貨號：
    CSB-MP342300DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    msl-3
  • Uniprot No.：
  • 別名：
    msl-3; CG8631; Protein male-specific lethal-3
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    The MSL proteins are essential for elevating transcription of the single X chromosome in the male (X chromosome dosage compensation). Mle, msl-1 and msl-3 are colocalized on the X chromosome. Each of the MSL proteins requires all the other MSLs for wild-type X-chromosome binding.
  • 基因功能參考文獻：
    1. MSL3 plays an important role in targeting the male specific lethal complex to chromatin in both humans and flies by binding to H4K20Me(1). PMID: 20943666
    2. A DNA-dependent interaction of MSL3 chromodomain with the H4 lysine20 monomethyl mark, was discovered. PMID: 20657587
    3. Nucleic acid binding determinants in the N terminus of MSL3 form an integrated domain which is required for interaction with MSL1 and localisation to X-chromosome. PMID: 15988010
    4. Localization to the male X chromosome and transcriptional upregulation of X-linked genes are two separable functions of the MSL-3 protein. PMID: 16547465
    5. These results provide strong evidence for both sequence-dependent and -independent steps in MSL targeting of dosage compensation to the male X chromosome. PMID: 18724933
    6. Using ChIP-chip analysis, the authors find that MSL3 chromodomain mutants retain binding to chromatin entry sites but show a clear disruption in the full pattern of MSL targeting in vivo, consistent with a loss of spreading. PMID: 19029895

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  • 亞細胞定位：
    Nucleus. Chromosome. Note=Msl-3 is associated with hundreds of discrete sites along the length of the X chromosome in males and not in females, and is also associated with 10-20 autosomal sites in males.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG8631

    STRING: 7227.FBpp0076635

    UniGene: Dm.2741



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