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  4. Recombinant Drosophila melanogaster Protein grainyhead (grh), partial

Recombinant Drosophila melanogaster Protein grainyhead (grh), partial

  • 中文名稱：
    黑腹果蠅grh重組蛋白
  • 貨號：
    CSB-YP319480DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅grh重組蛋白
  • 貨號：
    CSB-EP319480DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅grh重組蛋白
  • 貨號：
    CSB-EP319480DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅grh重組蛋白
  • 貨號：
    CSB-BP319480DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅grh重組蛋白
  • 貨號：
    CSB-MP319480DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    grh
  • Uniprot No.：
  • 別名：
    grh; elf1; CG5058Protein grainyhead; DNA-binding protein ELF-1; Element I-binding activity; Protein grainy-head; Transcription factor NTF-1
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Binds a CNS-specific regulatory element of the Dopa decarboxylase (Ddc) gene. Also interacts with sequences adjacent to other transcription units, including Ultrabithorax (Ubx) and engrailed (en). Activity in vivo may be required only at high levels transiently to activate the expression of Ddc in the CNS.
  • 基因功能參考文獻：
    1. Grh and Vvl participate in a regulatory network controlling epithelial maturation. PMID: 28760809
    2. Grh remains bound to at least 1146 genomic loci over days of development. Grh binds to target genes well before the Grh-dependent transcriptional program commences. PMID: 28007888
    3. The transcription factor Grainy head and the steroid hormone ecdysone drive the production of two partially overlapping sets of cuticle factors. PMID: 22458773
    4. results suggest that Grh acts as an activator to support expression of ind, which was surprising as we identified this factor using an element that mediates dorsally-localized repression PMID: 22216201
    5. Phosphorylation of Grainy head by ERK is essential for wound-dependent regeneration but not for development of an epidermal barrier PMID: 21187384
    6. Data show that apical membrane enlargement is critical for lumenal elongation of the Drosophila airways, and is independently controlled by the transcription factor Grainy head, whose activity is upregulated by Branchless. PMID: 12466193
    7. In the abdomen, Grh terminates neural proliferation by regulating the competence of neuroblasts to undergo apoptosis in response to Abdominal-A expression PMID: 16049114
    8. Grainy head is essential for post-embryonic neuroblasts to adopt the stem cell program appropriate to their position within the central nervous system; furthermore, ectopic expression of Grh is sufficient to promote ectopic E-cadherin. PMID: 16275038
    9. the grainyhead gene, which encodes a DNA binding protein, interacts with one Polycomb response element of the bithorax complex PMID: 16449654
    10. The level of Grh-mediated transcriptional wound responses is activated by Stit upon wounding coordinates cytoskeletal rearrangements. PMID: 19525935

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Grh/CP2 family, Grainyhead subfamily
  • 組織特異性：
    Restricted, during embryogenesis, to tissues derived from ectoderm, predominantly the central nervous system (CNS) and the epidermis.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG42311

    STRING: 7227.FBpp0288984

    UniGene: Dm.3595



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