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  4. Recombinant Drosophila melanogaster Probable tyrosyl-DNA phosphodiesterase (gkt), partial

Recombinant Drosophila melanogaster Probable tyrosyl-DNA phosphodiesterase (gkt), partial

  • 中文名稱(chēng)：
    黑腹果蠅gkt重組蛋白
  • 貨號(hào)：
    CSB-YP894723DLU
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    黑腹果蠅gkt重組蛋白
  • 貨號(hào)：
    CSB-EP894723DLU
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    黑腹果蠅gkt重組蛋白
  • 貨號(hào)：
    CSB-EP894723DLU-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    黑腹果蠅gkt重組蛋白
  • 貨號(hào)：
    CSB-BP894723DLU
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    黑腹果蠅gkt重組蛋白
  • 貨號(hào)：
    CSB-MP894723DLU
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    gkt
  • Uniprot No.：
  • 別名：
    gkt; Tdp1; CG8825; CG8826; Probable tyrosyl-DNA phosphodiesterase; Tyr-DNA phosphodiesterase; EC 3.1.4.-; Protein glaikit
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. May have low 3'exonuclease activity and may be able to remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. Required for normal polarization of epidermal cells, correct subcellular location of the Crb complex to the apical lateral membrane, and for normal neuronal development during embryonic development.
  • 基因功能參考文獻(xiàn)：
    1. a novel gene identified, glaikit, underlying the axonal bifurcation process PMID: 28465232
    2. study establishes that gkt is the Drosophila TDP1 gene, and that it is critical for neuroprotection, normal longevity, and repair of damaged DNA. PMID: 25331878
    3. glaikit (gkt) is essential for the formation of epithelial polarity and for neuronal development during Drosophila embryogenesis. PMID: 15556867
  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Tyrosyl-DNA phosphodiesterase family
  • 組織特異性：
    Expressed in the delaminating neuroblasts and a few ganglion mother cells in stage 11-14 embryonic central nervous system. Weak expression is seen in gonads at stage 16.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: dme:Dmel_CG8825

    STRING: 7227.FBpp0077263

    UniGene: Dm.3843



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