Recombinant Drosophila melanogaster Polyadenylate-binding protein (pAbp), partial

中文名稱：

黑腹果蠅pAbp重組蛋白
貨號：

CSB-YP325031DLU
規(guī)格：
來源：

Yeast
其他：

中文名稱：

黑腹果蠅pAbp重組蛋白
貨號：

CSB-EP325031DLU
規(guī)格：
來源：

E.coli
其他：

中文名稱：

黑腹果蠅pAbp重組蛋白
貨號：

CSB-EP325031DLU-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

黑腹果蠅pAbp重組蛋白
貨號：

CSB-BP325031DLU
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

黑腹果蠅pAbp重組蛋白
貨號：

CSB-MP325031DLU
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

pAbp
Uniprot No.：

P21187
別名：

pAbp; CG5119Polyadenylate-binding protein; PABP; Poly(A)-binding protein
種屬：

Drosophila melanogaster (Fruit fly)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Binds the poly(A) tail of mRNA. Since it interacts with the cap-associating translation initiation factor eIF4G, it is likely that it functions by linking Atx2 to the cap-binding complex. Forms a complex with tyf and Atx2 which functions in adult circadian pacemaker neurons to sustain circadian rhythms likely by switching between activator and repressor modes of post-transcriptional regulation via interaction with Lsm12a or me31B, respectively. The activator complex (Atx2-tyf activator complex) activates the TYF-dependent translation of per to maintain 24 hour periodicity in circadian locomotor behaviors, whereas the repressor complex (Atx2-Not1 repressor complex) promotes Not1-dependent post-transcriptional gene silencing and supports high-amplitude circadian rhythms in a per-independent manner. In 0-1 hour embryos, forms a ribonucleoprotein complex (RNP) with me31B, eIF4E1, tral and cup which binds to various mRNAs including maternal mRNAs, and downregulates their expression during the maternal-to-zygotic transition.
基因功能參考文獻：
1. PABP association with mRNA rapidly diminished with miRNA-induced silencing complex recruitment and before detectable deadenylation. PMID: 22635249
2. Single-molecule nanopore force spectroscopy was used to directly measure interactions between poly(A) and poly(A) binding protein. PMID: 22455926
3. PABP generally modulates poly(A) length and translation efficiency, neither PABP function nor GW182-PABP interaction is a prerequisite for these two silencing pathways. PMID: 22117217
4. Pabp binds to A-rich sequences (ARS) in the osk 3'UTR and these turned out to be required in vivo for osk function during early oogenesis. PMID: 21782810
5. These findings reveal that despite species-specific differences in the relative strength of the PABPC1-binding sites, the interaction between GW182 proteins and PABPC1 is critical for miRNA-mediated silencing in animal cells. PMID: 21063388
6. Cytoplasmic poly(A)-binding protein 1 (PABPC1) has a conserved role in nonsense-mediated mRNA decay. PMID: 17318186
7. Drosophila Larp associates with poly(A)-binding protein and is required for male fertility and syncytial embryo development PMID: 19631203
8. model proposed in which the GW182 silencing domain promotes translational repression, at least in part, by interfering with mRNA circularization and also recruits the deadenylase complex through the interaction with PABPC1 PMID: 19797087
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蛋白家族：

Polyadenylate-binding protein type-1 family
數(shù)據(jù)庫鏈接：

KEGG: dme:Dmel_CG5119

STRING: 7227.FBpp0085915

UniGene: Dm.7069