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  4. Recombinant Drosophila melanogaster Palmitoyl-protein thioesterase 1 (Ppt1)

Recombinant Drosophila melanogaster Palmitoyl-protein thioesterase 1 (Ppt1)

  • 中文名稱：
    黑腹果蠅Ppt1重組蛋白
  • 貨號(hào)：
    CSB-YP018587DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Ppt1重組蛋白
  • 貨號(hào)：
    CSB-EP018587DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Ppt1重組蛋白
  • 貨號(hào)：
    CSB-EP018587DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Ppt1重組蛋白
  • 貨號(hào)：
    CSB-BP018587DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Ppt1重組蛋白
  • 貨號(hào)：
    CSB-MP018587DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Ppt1; CG12108; Palmitoyl-protein thioesterase 1; PPT-1; EC 3.1.2.22; Palmitoyl-protein hydrolase 1
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    26-314
  • 氨基酸序列
    YIWWS PTKGGTNPEV LPVVLWHGMG DTCCVPFSLG AIMNLIVEQT KGGYVRSLQI GGNVLIDWQS GFFIHPNEQV DYVCKQLLQD EHLAKGYHAI GFSQGGQFLR AVAERCPNPP MRNLITLGGQ HQGIFGLPMC PTLTEKPCDY ITRLLDNAAY APEVQKALVQ ATYWHDPIME NKYRLGSTFL ADINNELFIN KFYIENLQKL KKFVMVQFLN DTIVQPKESQ WFQYYTTGQN KVIQPFTESK VYQDLGLDKM HRQGQLVFLG VEGDHLAISK AWFIQNIVPL LLEK
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides.
  • 基因功能參考文獻(xiàn)：
    1. Ppt1 has a role in synaptic vesicle exo- and endocytosis at motor nerve terminals of the Drosophila neuromuscular junction. PMID: 24091420
    2. Palmitoyl Protein Thioesterase 1 gene regulates neural specification and axon connectivity during Drosophila embryonic development PMID: 21203506
    3. An unbiased dominant loss-of-function genetic modifier screen is performed in Drosophila using a previously characterized Ppt1 gain-of-function system. PMID: 20206262
    4. major lysosomal thioesterase; Ppt2 expression cannot rescue neural inclusion phenotypes associated with loss of Ppt1 PMID: 18719403
    5. Increased levels of DmPpt1 lead to neurodegeneration possibly via a detrimentl effect on some aspect of PPT1's normal function. This suggests that the precise levels of PPT1 activity are important for neuronal cell survival. PMID: 14629778
    6. Ppt1 has a role in accumulation of storage material and in life span but not in preventing neurodegeneration PMID: 16452138
    7. Modifier genes identified in this screen connect Ppt1 function to synaptic vesicle cycling, endo-lysosomal trafficking, synaptic development, and activity-dependent remodeling of the synapse. PMID: 17409080

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  • 亞細(xì)胞定位：
    Lysosome.
  • 蛋白家族：
    Palmitoyl-protein thioesterase family
  • 組織特異性：
    Ubiquitously expressed.
  • 數(shù)據(jù)庫鏈接：


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