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  4. Recombinant Drosophila melanogaster Opsin Rh1 (ninaE), partial

Recombinant Drosophila melanogaster Opsin Rh1 (ninaE), partial

  • 中文名稱：
    黑腹果蠅ninaE重組蛋白
  • 貨號：
    CSB-YP356697DLU1
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅ninaE重組蛋白
  • 貨號：
    CSB-EP356697DLU1
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅ninaE重組蛋白
  • 貨號：
    CSB-EP356697DLU1-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅ninaE重組蛋白
  • 貨號：
    CSB-BP356697DLU1
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅ninaE重組蛋白
  • 貨號：
    CSB-MP356697DLU1
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ninaE
  • Uniprot No.：
  • 別名：
    ninaE; Rh1; CG4550; Opsin Rh1; Neither inactivation nor afterpotential E protein; Outer R1-R6 photoreceptor cells opsin
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.
  • 基因功能參考文獻：
    1. Organisms use multiple strategies to maximize visual capabilities in different light conditions. Many invertebrates show a daily cycle of shedding the photoreceptor's rhabdomeric membranes at dawn and rebuilding these during the following night. We show here that the Aedes aegypti mosquito possesses two distinct light-driven cellular signaling processes for modulating rhodopsin content during this cycle. PMID: 27798185
    2. Data show that site-directed mutagenesis of conserved residues has only modest effects on Rhodopsin 1 absorption. PMID: 26195627
    3. Data indicate that mutations in Golgi SNARE protein gos28 lead to defective rhodopsin (Rh1) trafficking. PMID: 25261468
    4. GPI biosynthesis is essential for rhodopsin sorting at the trans-Golgi network in Drosophila photoreceptors PMID: 23250212
    5. Our results indicate that fatp promotes photoreceptor survival by regulating Rh1 abundance PMID: 22844251
    6. Dominant rhodopsin (ninaE) mutants showed progressive age-dependent and light-independent loss of the deep pseudopupil. PMID: 22194648
    7. Data propose that Rh1 function in promoting RTW morphogenesis is not only needed to sustain apical membrane delivery but is also required for proper rhabdomeric membrane endocytosis and turnover. PMID: 21486953
    8. mutation of ninaE encoding Drosophila rhodopsin eliminates thermotactic discrimination in the comfortable temperature range; propose that rhodopsins represent a class of evolutionarily conserved GPCRs required for initiating thermosensory signaling PMID: 21393546
    9. Data show tat mutant flies bearing certain ninaE alleles have dramatically reduced rhodopsin-1 protein levels. PMID: 19805114
    10. expression of ceramidase facilitates the dissolution of incompletely formed and inappropriately located elements of rhabdomeric membranes in ninaE(I17) mutants lacking the G protein receptor Rh1 in R1-R6 photoreceptor cells PMID: 14769922
    11. NinaE(pp100) represented the first rhodopsin mutation that stabilized rhodopsin-arrestin complex and led to elevated levels of G(q)alpha in the cytosol, which mediated a novel retinal degeneration pathway PMID: 15014127
    12. We present evidence that the Drosophila GBS regulatory element (Glass-binding site), derived from the Rh1 rhodopsin gene, is able to drive in vivo eye-specific expression of a Ccwhite+ transgene in the Mediterranean fruitfly Ceratitis capitata. PMID: 16756553
    13. Data show that the double Rhodopsin 1 (ninaE) and Rhodopsin mutant was not entrained to red light. PMID: 18766027

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  • 亞細胞定位：
    Membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Opsin subfamily
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG4550

    STRING: 7227.FBpp0083266

    UniGene: Dm.6744



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