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  4. Recombinant Drosophila melanogaster Neuropeptide F (npf)

Recombinant Drosophila melanogaster Neuropeptide F (npf)

  • 中文名稱：
    黑腹果蠅NPF重組蛋白
  • 貨號：
    CSB-YP893268DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅NPF重組蛋白
  • 貨號：
    CSB-EP893268DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅NPF重組蛋白
  • 貨號：
    CSB-EP893268DLU-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅NPF重組蛋白
  • 貨號：
    CSB-BP893268DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅NPF重組蛋白
  • 貨號：
    CSB-MP893268DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NPF
  • Uniprot No.：
  • 別名：
    NPF; CG10342; Neuropeptide F; NPF; dNPF
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Cytoplasmic domain
  • 表達(dá)區(qū)域：
    35-62
  • 氨基酸序列
    NDVNTM ADAYKFLQDL DTYYGDRARV RF
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Integral part of the sensory system that mediates food signaling, providing the neural basis for the regulation of food response; coordinates larval foraging and social behavior changes during development. Required in dopaminergic (DA) neurons that innervate the mushroom body for satiety to suppress appetitive memory performance; a key factor in the internal state of hunger in the brain. NPF neurons coordinately modulate diverse sensory and motor neurons important for feeding, flight, and locomotion. NPF/NPFR pathway exerts its suppressive effect on larval aversion to diverse stressful stimuli (chemical stress and noxious heat) through attenuation of TRP channel-induced neuronal excitation. NPF neural signaling system plays a physiological role in acute modulation of alcohol sensitivity in adults, rather than a general response to intoxication by sedative agents. Activation and inhibition of the NPF system reduces and enhances ethanol preference, respectively. Sexual experience, the NPF system activity and ethanol consumption are all linked; sexual deprivation is a major contributor to enhanced ethanol preference.
  • 基因功能參考文獻(xiàn)：
    1. Flies in which neuropeptide F (NPF) neurons are activated display preference for the illuminated side of the chamber. We show that optogenetic activation of NPF neuron is rewarding in olfactory conditioning experiments and that the preference for NPF neuron activation is dependent on NPF signaling. PMID: 28874527
    2. NPF-expressing cells, specifically those expressing the circadian photoreceptor cryptochrome, are largely responsible for changes to sleep behavior caused by NPF neuron activation PMID: 28636933
    3. In Drosophila melanogaster neuropeptide F (NPF) modulates the responses of a specific population of antennal olfactory sensory neurons (OSNs) to food-derived odors. Knock-down of NPF in NPF neurons specifically reduces the responses of the ab3A neurons to ethyl butyrate, a volatile ester found in apples and other fruits. PMID: 28482894
    4. These data highlight the importance of neuronal clocks for peripheral rhythms, particularly in a specific detoxification pathway, and identify a novel and conserved role for NPF/Npy in circadian rhythms. PMID: 27077948
    5. Too much or too little NPF or its shorter isoform, which are key players in homeostatic feeding regulation, leads to increased sensitivity to amino acid starvation and diminished survivorship when compared to controls. PMID: 26909968
    6. NPF signaling is involved in the circadian locomotor rhythm in light:dark cycles. PMID: 23614491
    7. This study identify a small subset of clock neurons in the male brain that regulate rival-induced Longer-Mating-Duration via neuropeptide signaling. PMID: 24314729
    8. Neuropeptide F-positive clock neurons are involved in both the control of the free-running period in constant darkness and the phasing and amplitude of the evening activity in light-dark cycles. PMID: 21826659
    9. Data show that neuropeptide F (NPF)expression is regulated in both sex-nonspecific and male-specific manners, and that NPF also plays a role in clock-controlled sexual dimorphism in adult Drosophila. PMID: 16894172
    10. Silencing of the neuropeptide F (npf) circuit increases fly aggression, demonstrating an opposite modulation to 5-HT. PMID: 17450142
    11. These findings reveal an uncharacterized role of PKC in NPY/NPF-mediated acute ethanol sensitivity in flies and possibly mammals. PMID: 18675322

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    NPY family
  • 組織特異性：
    Expressed in midgut, brain lobes and ventral nerve cord of larvae. Predominantly expressed in two pairs of protocerebral neurons in the larval CNS (at protein level). Intense expression is also seen in the fan-shaped body of the central complex and two la
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG10342

    STRING: 7227.FBpp0304074

    UniGene: Dm.5729



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