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  4. Recombinant Drosophila melanogaster Netrin-B (NetB), partial

Recombinant Drosophila melanogaster Netrin-B (NetB), partial

  • 中文名稱：
    黑腹果蠅NetB重組蛋白
  • 貨號：
    CSB-YP638767DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅NetB重組蛋白
  • 貨號：
    CSB-EP638767DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅NetB重組蛋白
  • 貨號：
    CSB-EP638767DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅NetB重組蛋白
  • 貨號：
    CSB-BP638767DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅NetB重組蛋白
  • 貨號：
    CSB-MP638767DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NetB
  • Uniprot No.：
  • 別名：
    NetB; CG10521Netrin-B
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either fra or unc-5 receptors will lead to axon attraction or repulsion, respectively. While short-range repulsion requires both fra and unc-5 receptors, long-range repulsion only requires unc-5.
  • 基因功能參考文獻：
    1. Data find that Abl interacts both physically and genetically with the Netrin receptor Frazzled, and that disrupting this interaction prevents Abl from promoting midline axon crossing. PMID: 23720041
    2. Netrin-B is specifically localized in photoreceptor axons owing to axonal release by lamina neurons L3 and capture by target neuron-associated Frazzled. PMID: 22794263
    3. Dscam1 functions to counter Drosophila sensory neuron dendritic targeting signals provided by secreted Netrin-B and Frazzled, a netrin receptor. Loss of Dscam1 function resulted in aberrant dendrite accumulation at a Netrin-B-expressing target. PMID: 21871804
    4. Dscam proteins function as attractive receptors for Netrin and also act in parallel to Frazzled/DCC. PMID: 18948420
  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix. Cytoplasm, perinuclear region.
  • 組織特異性：
    At 24 hr after puparium formation (APF), detected in the most anterior (oldest) L3, L4 and L5 lamina neurons (at protein level). At 48 hr APF, expressed in all L3, L4 and L5 neurons with slightly higher expression in the L3 neurons (at protein level). At
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG10521

    STRING: 7227.FBpp0291228

    UniGene: Dm.3913



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