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  4. Recombinant Drosophila melanogaster Netrin-A (NetA), partial

Recombinant Drosophila melanogaster Netrin-A (NetA), partial

  • 中文名稱：
    黑腹果蠅NetA重組蛋白
  • 貨號：
    CSB-YP634512DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅NetA重組蛋白
  • 貨號：
    CSB-EP634512DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅NetA重組蛋白
  • 貨號：
    CSB-EP634512DLU-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅NetA重組蛋白
  • 貨號：
    CSB-BP634512DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅NetA重組蛋白
  • 貨號：
    CSB-MP634512DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NetA
  • Uniprot No.：
  • 別名：
    NetA; CG18657; Netrin-A
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Netrins control guidance of CNS commissural axons at the midline and peripheral motor axons to their target muscles.
  • 基因功能參考文獻(xiàn)：
    1. mutation of mushroom body defect (mud) dramatically enhances the phenotype of Netrin or frazzled mutants, resulting in many more axons failing to cross the midline, although mutations in mud alone have little effect. PMID: 26893348
    2. Contact-mediated cell adhesion via Fmi acts in addition to the Net/Fra guidance system during axon pathfinding across the midline, underlining the importance of PCP molecules during vertebrates and invertebrates midline development. PMID: 25440577
    3. adult Drosophila lacking both netrin genes, NetA and NetB, have fertility defects in both sexes together with an inability to fly and reduced viability. PMID: 23977313
    4. In peripodial cells Frazzled is endocytosed and undergoes NetA-dependent degradation, which is required for eversion. PMID: 24225841
    5. These data are the first to show that Netrin and Frazzled regulate the placement of gap junctions presynaptically at a synapse. PMID: 24741033
    6. NetA and semaphorin-1a can specifically promote growth through induction of key cellular growth regulators. PMID: 20162636
    7. Misexpression of Netrin-A in oenocytes, secretory cells that lie ventral to the cap cell, leads to aberrant cap cell morphogenesis, suggesting that Netrin-A acts as an instructive cue to direct the growth of the cap cell process. PMID: 20530550
    8. NETRIN serves as a chemo-attractant while SLIT functions antagonistically to NETRIN as a chemo-repellent during embryonic salivary gland migration parallel to the CNS in Drosophila. PMID: 15950216
    9. before reaching the segmental boundary, the longitudinal axons' responsiveness to Netrin presented on the commissure is suppressed by Roundabout, through counteracting Gq signaling PMID: 16341212
    10. the midline signalling systems Slit/Robo and Netrin/Frazzled are the main molecular mechanisms that underlie dendritic targeting with respect to the midline. PMID: 19771146
    11. the medio-lateral positioning of motoneuron dendrites in the leg neuropil is controlled by the midline signalling systems Slit-Robo and Netrin-Fra. PMID: 19771147

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  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 組織特異性：
    At the midline of developing CNS at the time of commissure formation and in different subsets of neurons, muscles, and epidermal patches.
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG18657

    STRING: 7227.FBpp0073759

    UniGene: Dm.2915



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