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  4. Recombinant Drosophila melanogaster Myosin heavy chain, muscle (Mhc), partial

Recombinant Drosophila melanogaster Myosin heavy chain, muscle (Mhc), partial

  • 中文名稱：
    黑腹果蠅Mhc重組蛋白
  • 貨號：
    CSB-YP361583DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Mhc重組蛋白
  • 貨號：
    CSB-EP361583DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Mhc重組蛋白
  • 貨號：
    CSB-EP361583DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Mhc重組蛋白
  • 貨號：
    CSB-BP361583DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Mhc重組蛋白
  • 貨號：
    CSB-MP361583DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Mhc
  • Uniprot No.：
  • 別名：
    Mhc; CG17927; Myosin heavy chain; muscle
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Muscle contraction.
  • 基因功能參考文獻：
    1. Unipolar distributions of junctional Myosin II identify cell stripe boundaries that drive cell intercalation throughout Drosophila axis extension. PMID: 27183005
    2. We conclude that MHC isoforms can alter FSA and hence cyclical power generation but that isoforms can only endow a muscle type with moderate FSA Highly SA muscle types, such as IFM, likely use a different or additional mechanism. PMID: 27881413
    3. Introduction of a mutation (R759E) in the converter domain inhibits the steady-state ATPase of myosin S1, whereas an additional mutation in the relay domain (N509K) is able to restore the ATPase. PMID: 26586917
    4. The vector of cell movement is regulated by localised epidermal growth factor (EGF) signalling from the distally placed tip cell lineage and the acquisition of planar polarity leads to asymmetric pulsatile Myosin II accumulation. PMID: 25460353
    5. Using transgenic techniques the study shows that an embryonic myosin converter domain influences Drosophila indirect flight muscle stretch activation, power generation and flight. PMID: 24115062
    6. Data indicate that disruption of the relay-converter domain interface leads to complete absence of myosin ATPase activity. PMID: 24627474
    7. The abnormalities observed in myosin function and muscle ultrastructure likely contribute to muscle weakness observed in our flies and patients. PMID: 22496423
    8. The data indicated that interactions of the relay and converter of myosin heavy chain during the contractile cycle are key to myosin-isoform-specific kinetic and mechanical functions. PMID: 22226837
    9. These results provide the first mutational evidence that intramolecular signaling between the relay loop and converter domain of mhc is critical for myosin function both in vitro and in muscle. PMID: 20362584
    10. function of different myosin isoforms PMID: 11901423
    11. myosin rod protein has an active function to modulate the contractile activity of muscles in which it is expressed PMID: 12927543
    12. Single point mutations in the ATP binding/hydrolysis domain of Mhc and lead to degeneration of the flight muscles. PMID: 15051736
    13. the N-terminal region is important in tuning myosin kinetics to match muscle speed for optimal locomotory performance PMID: 15345559
    14. the exon 7 domain participates in the regulation of the attachment of myosin to actin in order to fine-tune the physiological properties of Drosophila myosin isoforms PMID: 16154586
    15. exon 7 plays a substantial role in establishing fiber speed and flight performance, and the limiting step that sets ATPase rate in Drosophila myosin has little to no direct influence in setting fmax for fast muscle fiber types PMID: 16399836
    16. Myosin II (zipper) and fringe have roles in planar polarization of the denticle field in the Drosophila embryo PMID: 16890930
    17. Study shows that a 2.6kb fragment of the muscle myosin heavy-chain gene of Drosophila melanogaster (containing 458 base pairs of upstream sequence, the first exon, the first intron and the beginning of the second exon) drives expression in all muscles. PMID: 17194628
    18. Alternative hinges are important in dictating the distinct functional properties of myosin isoforms and the muscles in which they are expressed. PMID: 17316684

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  • 亞細胞定位：
    Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
  • 組織特異性：
    Expressed in larval and adult muscles. Isoforms containing exon 9a are expressed in indirect flight muscles, exons 9a and 9b are expressed in jump muscles, exons 9b and 9c are expressed in other larval and adult muscles.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG17927

    STRING: 7227.FBpp0080452

    UniGene: Dm.2761



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