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  4. Recombinant Drosophila melanogaster Myosin-IA (Myo31DF), partial

Recombinant Drosophila melanogaster Myosin-IA (Myo31DF), partial

  • 中文名稱(chēng)：
    黑腹果蠅Myo31DF重組蛋白
  • 貨號(hào)：
    CSB-YP632570DLU
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    黑腹果蠅Myo31DF重組蛋白
  • 貨號(hào)：
    CSB-EP632570DLU
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    黑腹果蠅Myo31DF重組蛋白
  • 貨號(hào)：
    CSB-EP632570DLU-B
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    黑腹果蠅Myo31DF重組蛋白
  • 貨號(hào)：
    CSB-BP632570DLU
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    黑腹果蠅Myo31DF重組蛋白
  • 貨號(hào)：
    CSB-MP632570DLU
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Myo31DF
  • Uniprot No.：
  • 別名：
    Myo31DF; Myo1D; CG7438; Unconventional myosin ID; MyoID; Brush border myosin IA; BBMIA; Myosin 1D; Myo1D; Myosin-IA; MIA; MyoIA
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Unconventional myosin that functions as actin-based motor protein with ATPase activity. Binds to membranes enriched in phosphatidylinositol 4-5-bisphosphate, and can glide along actin filaments when anchored to a lipid bilayer. Generates left-right asymmetry at the level of single cells, organs and the whole body via its interaction with the actin cytoskeleton, both in the embryo and the adult. Normal left-right asymmetry of the larval midgut and hindgut requires expression in the embryonic hindgut epithelium during a critical time period, 10 to 12.75 hours after egg laying. This period corresponds to a late stage of germband retraction, and precedes left-right asymmetric morphogenesis. Expression in segment H1 of the imaginal ring is required at 0 to 24 hours after pupation for changes of cell shape and orientation in the H2 segment, which then gives rise to normal, dextral looping of the adult hindgut. Required during a critical period, 126-132 hours after egg laying, for normal, dextral rotation of the adult male genitalia. Has a double role by promoting dextral rotation in the posterior compartment of segment A8 of the male genital disk, and in repressing sinistral looping in the anterior compartment.
  • 基因功能參考文獻(xiàn)：
    1. Myo31DF-overexpressing cells suggest that cell-shape chirality was established in each cell and reflects intrinsic planar cell chirality. PMID: 24800645
    2. DE-Cadherin regulates unconventional Myosin ID and Myosin IC in Drosophila left-right asymmetry establishment. PMID: 22491943
    3. left-right(LR)directional rotation of hindgut epithelial tube; DECad is distributed to cell boundaries with LR asymmetry which is responsible for planar cell-shape chirality formation; myosin ID switches LR polarity found in PCC and in DE-Cad distribution PMID: 21764746
    4. the actin cytoskeleton and myosin I proteins may be crucial for generating left-right asymmetry in invertebrates PMID: 16598258
    5. Myo31DF interacts and colocalizes with beta-catenin, suggesting that situs inversus genes can direct left-right development through the adherens junction PMID: 16598259
    6. the organ specificities of the Myo31DF and Myo61F activities depended on their head regions PMID: 18521948

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  • 亞細(xì)胞定位：
    Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Cell junction, adherens junction. Cell projection.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
  • 組織特異性：
    In the embryo, expressed in gastric caeca, midgut cells of the proventriculus, and in the mid and hindgut. In the larval gut brush border, expression is in the terminal web domain. In the adult gut brush border, expression remains in the web domain and ha
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: dme:Dmel_CG7438

    STRING: 7227.FBpp0079625

    UniGene: Dm.5426



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