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  4. Recombinant Drosophila melanogaster Myocyte-specific enhancer factor 2 (Mef2), partial

Recombinant Drosophila melanogaster Myocyte-specific enhancer factor 2 (Mef2), partial

  • 中文名稱：
    黑腹果蠅Mef2重組蛋白
  • 貨號：
    CSB-YP336782DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Mef2重組蛋白
  • 貨號：
    CSB-EP336782DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Mef2重組蛋白
  • 貨號：
    CSB-EP336782DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Mef2重組蛋白
  • 貨號：
    CSB-BP336782DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Mef2重組蛋白
  • 貨號：
    CSB-MP336782DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Mef2
  • Uniprot No.：
  • 別名：
    Mef2; CG1429Myocyte-specific enhancer factor 2; D-mef2; MADS domain transcription factor
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcription factor that could be a key player in early mesoderm differentiation and may be required for subsequent cell fate specifications within the somatic and visceral/heart mesodermal layers. Essential for myoblast fusion and consequently muscle formation in adults. During embryonic and pupal development, binds to the enhancer of the myoblast fusion gene sing and activates its transcription.
  • 基因功能參考文獻：
    1. Overexpression of Tinman and Pannier resulted in 20% of embryos with ectopic Hand and Sur expression. By adding MEF2 alongside Tinman and Pannier, an expansion in expression of Hand and Sur was observed. PMID: 26225919
    2. Our results show that Mef2 and possibly Sd regulate a Dl enhancer specifically expressed in the developing indirect flight muscles and that Mef2 is required for Dl expression in developing indirect flight muscles. PMID: 25247309
    3. critical switch between anabolic and immune function in the adult Drosophila fat body [review] PMID: 25483252
    4. MEF2 directly controls fusion at multiple stages of the life cycle PMID: 25797154
    5. The loss of phosphorylated MEF2 contributes to loss of anabolic enzyme expression in Gram-negative bacterial infection. MEF2 is thus a critical transcriptional switch in the adult fat body between metabolism and immunity. PMID: 24075010
    6. Mutation of MEF2 sites severely impaired both spontaneous and induced expression of not only BZLF1, but also BRLF1 in comparison to wild-type or revertant virus cases. PMID: 23843637
    7. Mef2 transmits circadian clock information in neuronal plasticity, which contributes to locomotor activity rhythms. PMID: 23889933
    8. The negative feedback circuit between miR-92b and Mef2 efficiently maintains the stable expression of both components that is required for homeostasis during Drosophila muscle development. PMID: 22899845
    9. transcription factor Mef2 has multiple roles in adult Drosophila musculature formation PMID: 22357930
    10. MEF2 remains an essential factor, participating in control of myoblast fusion, and myofibrillogenesis in developing myotubes. PMID: 22008792
    11. Lmd is a tissue-specific modulator of Mef2 activity, acting as both a transcriptional activator and repressor. PMID: 20617173
    12. a normal level of Mef2 activity is required in clock neurons to maintain robust and accurate circadian behavioral rhythms PMID: 20427646
    13. Temperature sensitivity for mutations affecting the Drosophila muscle regulator Myocyte enhancer factor-2. PMID: 19564485
    14. Drosophila MEF2 is a direct regulator of Actin57B transcription in cardiac, skeletal, and visceral muscle lineages. PMID: 11744367
    15. Adult myogenesis in Drosophila can proceed independently of Mef2. PMID: 15956678
    16. expression of the muscle differentiation gene Myocyte enhancer factor-2 (Mef2) is normally delayed in twist-expressing adult myoblasts until the end of the third larval instar and it is an ecdysone-dependent event PMID: 16325168
    17. the use of ChEST in Drosophila to identify several previously unknown targets of myocyte enhancer factor 2 (MEF2), a key regulator of myogenic differentiation PMID: 16339902
    18. mef2 directly regulates target genes at all stages of muscle development. PMID: 16740481
    19. suggests a route by which mef2 can orchestrate the muscle differentiation program and contribute to the stringent regulation of gene expression during myogenesis PMID: 18198273

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    MEF2 family
  • 組織特異性：
    Expressed in all presumptive mesoderm prior to the splitting process that generates the somatic and visceral/ heart mesoderm. After the subdivision, it is found in both the somatic and the visceral/heart mesoderm.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG1429

    UniGene: Dm.7054



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