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  4. Recombinant Drosophila melanogaster Mesencephalic astrocyte-derived neurotrophic factor homolog (Manf)

Recombinant Drosophila melanogaster Mesencephalic astrocyte-derived neurotrophic factor homolog (Manf)

  • 中文名稱：
    黑腹果蠅Manf重組蛋白
  • 貨號：
    CSB-YP896451DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Manf重組蛋白
  • 貨號：
    CSB-EP896451DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Manf重組蛋白
  • 貨號：
    CSB-EP896451DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Manf重組蛋白
  • 貨號：
    CSB-BP896451DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Manf重組蛋白
  • 貨號：
    CSB-MP896451DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Manf
  • Uniprot No.：
  • 別名：
    Manf; ARP-like; CG7013; Mesencephalic astrocyte-derived neurotrophic factor homolog; DmMANF; MANF/CDNF-like protein
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    23-173
  • 氨基酸序列
    LKEEDCEV CVKTVRRFAD SLDDSTKKDY KQIETAFKKF CKAQKNKEHR FCYYLGGLEE SATGILNELS KPLSWSMPAE KICEKLKKKD AQICDLRYEK QIDLNSVDLK KLKVRDLKKI LNDWDESCDG CLEKGDFIKR IEELKPKYSR SEL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required during the maturation of the embryonic nervous system for maintenance of neuronal and cuticular connectivity. Essential for maintenance of dopaminergic neurons and dopamine levels.
  • 基因功能參考文獻：
    1. Study employed the Drosophila genetics to screen for potential interaction partners of Drosophila Manf (DmManf) in vivo; show that DmManf plays a role in the development of Drosophila wing. PMID: 28578657
    2. These data suggest a role for Manf in the regulation of Drosophila unfolded protein response. PMID: 26975047
    3. DmMANF expression in the adult brain is much wider than in the embryonic and larval stages. It is expressed in both glia and neurons including dopaminergic neurons. PMID: 25917377
    4. study offers further insights into the functional conservation between invertebrate and mammalian MANF/CDNF proteins and reveals the importance of the C-terminal domain for MANF activity in vivo PMID: 24019940
    5. role of MANF in multicellular organism development PMID: 22494833
    6. DmMANF is an invertebrate neurotrophic factor supporting dopaminergic neurons. PMID: 19164766

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    ARMET family
  • 組織特異性：
    In stage 12, expression is seen in the stomodeum and the salivary gland primordia, and weakly in the mesoderm. From stage 13 onward, expression is in Garland cells, the outer wall of the proventriculus and salivary glands. Punctuate expression is also in
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG7013

    STRING: 7227.FBpp0082735

    UniGene: Dm.3726



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