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  4. Recombinant Drosophila melanogaster Kinetochore protein Spc25 (Spc25)

Recombinant Drosophila melanogaster Kinetochore protein Spc25 (Spc25)

  • 中文名稱：
    黑腹果蠅Spc25重組蛋白
  • 貨號：
    CSB-YP892679DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Spc25重組蛋白
  • 貨號：
    CSB-EP892679DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Spc25重組蛋白
  • 貨號：
    CSB-EP892679DLU-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Spc25重組蛋白
  • 貨號：
    CSB-BP892679DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Spc25重組蛋白
  • 貨號：
    CSB-MP892679DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Spc25
  • Uniprot No.：
  • 別名：
    Spc25; Cal2; mitch; CG7242; Kinetochore protein Spc25; Chromosome alignment defect 2; Mitotic chaos
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-222
  • 氨基酸序列
    MAIIMTESSY ERRVKALYEK QIHMEALEAK FIKKVFKFNS NLLDVKEAAS RHQRKVGKLQ KVLMERREEL DKRVSFIEEL DRELEATKLH NLAMKDWFKQ QKMLAKQRKN EIMESIHTLS KTTRTYINQE ALPARVKGVT VLRGDKRDQL IPFDLKATDV EGLDSLCQHL ESLNVDVAQW QQLISLAMDM AMESRAPTTP PKEADNCKSI IEIDLTSPMS HT
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Acts as a component of the essential kinetochore-associated Ndc80 complex, which is required for chromosome segregation and spindle checkpoint activity during meiosis and mitosis. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. Participates in SAC signaling that responds specifically to disruptions in spindle microtubule dynamics. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
  • 基因功能參考文獻：
    1. mitch gene corresponds to CG7242 & encodes possible ortholog of the Spc24 or Spc25 subunit of Ndc80 kinetochore complex; data suggest Mitch participates in spindle assembly checkpoint signaling that responds to disruptions in spindle microtubule dynamics PMID: 17895365
  • 亞細胞定位：
    Nucleus. Chromosome, centromere, kinetochore.
  • 蛋白家族：
    SPC25 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG7242

    STRING: 7227.FBpp0082158

    UniGene: Dm.3322



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