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  4. Recombinant Drosophila melanogaster Inactivation-no-after-potential D protein (inaD), partial

Recombinant Drosophila melanogaster Inactivation-no-after-potential D protein (inaD), partial

  • 中文名稱：
    黑腹果蠅inaD重組蛋白
  • 貨號：
    CSB-YP638752DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅inaD重組蛋白
  • 貨號：
    CSB-EP638752DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅inaD重組蛋白
  • 貨號：
    CSB-EP638752DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅inaD重組蛋白
  • 貨號：
    CSB-BP638752DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅inaD重組蛋白
  • 貨號：
    CSB-MP638752DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    inaD
  • Uniprot No.：
  • 別名：
    inaD; CG3504; Inactivation-no-after-potential D protein
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in the negative feedback regulation of the light-activated signaling cascade in photoreceptors through a calcium-mediated process. Interacts with tetrapeptide ligand located in C-terminal sequence of 3 key components of the visual cascade, tethering them and forming a macromolecular signaling phototransduction complex.
  • 基因功能參考文獻：
    1. show that INAD PDZ3 binds to the TRP channel tail with a new mechanism. A 15-residue C-terminal tail of TRP forms a b hairpin structure and binds to a surface of INAD PDZ3 outside the canonical target-binding aB/bB groove. Amino acid residues from both strands of the TRP b hairpin are engaged in binding, and thus the INAD PDZ3/TRP interaction is highly specific and with high affinity. PMID: 26853938
    2. Through biochemical and structural studies, study shows that the redox potential of PDZ5 is allosterically regulated by its interaction with another INAD domain, PDZ4. PMID: 21703451
    3. genetic screen of the third chromosome for mutations that result in mislocalization of an INAD-GFP fusion protein PMID: 20404479
    4. study demonstrates conformational switch mechanism for PDZ domain function & suggest INAD behaves more like a dynamic machine rather than a passive scaffold, regulating signal transduction through cycles of conformational change PMID: 17923089
    5. TRP associates with INAD via its last 14 residues to facilitate its regulation by eye-PKC that fine-tunes the visual signaling PMID: 18036153

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  • 亞細胞定位：
    Cell membrane; Peripheral membrane protein.
  • 組織特異性：
    Expressed in rhabdomeres of the compound eyes and ocelli.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG3504

    STRING: 7227.FBpp0293662

    UniGene: Dm.2964



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