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  4. Recombinant Drosophila melanogaster Histone-lysine N-methyltransferase pr-set7 (pr-set7), partial

Recombinant Drosophila melanogaster Histone-lysine N-methyltransferase pr-set7 (pr-set7), partial

  • 中文名稱：
    黑腹果蠅pr-set7重組蛋白
  • 貨號：
    CSB-YP896087DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅pr-set7重組蛋白
  • 貨號：
    CSB-EP896087DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅pr-set7重組蛋白
  • 貨號：
    CSB-EP896087DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅pr-set7重組蛋白
  • 貨號：
    CSB-BP896087DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅pr-set7重組蛋白
  • 貨號：
    CSB-MP896087DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    pr-set7
  • Uniprot No.：
  • 別名：
    PR-Set7; KMT5A; CG3307Histone-lysine N-methyltransferase PR-Set7; EC 2.1.1.361; Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07; dSET8
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20' monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, possibly by contributing to the maintenance of proper higher-order structure of DNA and chromosome condensation during mitosis.
  • 基因功能參考文獻：
    1. coincident with the ATR-dependent cell cycle arrest, increased DNA damage is specifically limited to late replicating regions of the Drosophila genome, suggesting that PR-Set7-mediated monomethylation of H4K20 is critical for maintaining the genomic integrity of late replicating domains PMID: 27131378
    2. Results support the hypothesis that methylation of H4 lysine 20 by PR/Set7 maintains silent chromatin, in part, by precluding neighboring acetylation on the H4 tail PMID: 12086618
    3. crystal structure reveals the basis of the specificity of the enzyme for histone H4-K20 PMID: 15933069
  • 亞細胞定位：
    Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes. Associates to chromatin-dense and transcriptionally silent euchromatic regions.
  • 蛋白家族：
    Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, PR/SET subfamily
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG3307

    STRING: 7227.FBpp0082387

    UniGene: Dm.36781



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