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  4. Recombinant Drosophila melanogaster Histone-lysine N-methyltransferase eggless (egg), partial

Recombinant Drosophila melanogaster Histone-lysine N-methyltransferase eggless (egg), partial

  • 中文名稱：
    黑腹果蠅egg重組蛋白
  • 貨號：
    CSB-YP654157DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅egg重組蛋白
  • 貨號：
    CSB-EP654157DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅egg重組蛋白
  • 貨號：
    CSB-EP654157DLU-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅egg重組蛋白
  • 貨號：
    CSB-BP654157DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅egg重組蛋白
  • 貨號：
    CSB-MP654157DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    egg
  • Uniprot No.：
  • 別名：
    egg; CG12196; Histone-lysine N-methyltransferase eggless; EC 2.1.1.355; SETDB1 homolog
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 in ovary. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting Su(var)205/HP1 to methylated histones. Plays a central role during oogenesis.
  • 基因功能參考文獻：
    1. Study identified a H3K9 methyltransferase, SETDB1/eggless, a Drosophila homologue of the mammalian SETDB1, which confers the robustness for the precise determination of bristle number. PMID: 26914287
    2. Mosaic analysis also reveals that the early egg chamber budding defects in egg- ovaries are due to an intrinsic requirement for egg in follicle stem cells and their descendents PMID: 24485852
    3. The results showed that on chromosome 4, Su(var)3-9, SETDB1 and HP1a target the same genes. PMID: 23476027
    4. dSet1 is responsible for the bulk of H3K4 di- and trimethylation throughout Drosophila development. PMID: 22048023
    5. this study has revealed the essential roles of Eggless (Egg), a H3K9 methyltransferase in controlling stem cell maintenance and differentiation through distinct mechanisms. PMID: 22216012
    6. egg is required in the ovary for trimethylation of histone H3 at its K9 residue. PMID: 17164421
    7. results indicate that dSETDB1 and SU(VAR)3-9 act together with distinct roles during oogenesis, with dsetdb1 being of particular importance due to its germline stem cell-specific function and more severe mutant phenotype PMID: 18493619
    8. The genetic interactions between dSETDB1 and Su(var)3-9 mutations emphasize the importance of maintaining the activities of these histone methyltransferases in balance for normal genome function. PMID: 19189944
    9. Together with Egg, Wde localizes to euchromatin, is enriched on chromosome 4, and binds to the Painting of fourth (POF) protein. PMID: 19750210

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  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar3-9 subfamily
  • 組織特異性：
    Expressed in ovary (at protein level).
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG12196

    STRING: 7227.FBpp0111689

    UniGene: Dm.14397



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