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  4. Recombinant Drosophila melanogaster Histone chaperone asf1 (asf1)

Recombinant Drosophila melanogaster Histone chaperone asf1 (asf1)

  • 中文名稱：
    Recombinant Drosophila melanogaster Histone chaperone asf1(asf1)
  • 貨號：
    CSB-YP896044DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Histone chaperone asf1(asf1)
  • 貨號：
    CSB-EP896044DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Histone chaperone asf1(asf1)
  • 貨號：
    CSB-EP896044DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Histone chaperone asf1(asf1)
  • 貨號：
    CSB-BP896044DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Histone chaperone asf1(asf1)
  • 貨號：
    CSB-MP896044DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    asf1
  • Uniprot No.：
  • 別名：
    asf1; CG9383Histone chaperone asf1; Anti-silencing function protein 1; Replication-coupling assembly factor subunit ASF1; RCAF subunit ASF1; dASF1
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-218
  • 氨基酸序列
    MAKVHITNVV VLDNPSSFFN PFQFELTFEC IEELKEDLEW KMIYVGSAES EEHDQVLDTI YVGPVPEGRH IFVFQADPPD VSKIPEPDAV GVTIVLLTCS YRGQEFVRVG YYVNNDYADP EMRENPPTKP LFEKLTRNIL ASKPRVTRFK INWDYGHING NGNGVENGHQ DEMATDGPST SEAASAVIHP EDDNSLAMPM ENGIKALNEN SNSLAMEC
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Plays a role in the formation of silent heterochromatin.
  • 基因功能參考文獻：
    1. ASF1 is critically required to load H3.3-H4 dimers on the HIRA complex prior to histone deposition on paternal DNA. This separation of tasks could optimize the rapid assembly of paternal chromatin within the gigantic volume of the egg cell. In contrast, ASF1 is surprisingly dispensable for the amplification of cleavage nuclei, although chromatin integrity is likely compromised in asf1 knocked-down embryos. PMID: 29751847
    2. ASF1 cellular levels are tightly controlled by distinct pathways and provide a molecular mechanism for post-translational regulation of dASF1 and hASF1a by TLK kinases. PMID: 20016786
    3. dASF1 has a direct role in modifying chromatin structure during DNA replication and this function of dASF1 is important for the processivity of the replication machinery. PMID: 16396992
    4. Asf1 is directed via interactions with sequence-specific complexes to mediate silencing of specific target genes. PMID: 17925233
  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    ASF1 family
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG9383

    STRING: 7227.FBpp0074715

    UniGene: Dm.2521



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