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  4. Recombinant Drosophila melanogaster Heparan sulfate 2-O-sulfotransferase pipe (pip), partial

Recombinant Drosophila melanogaster Heparan sulfate 2-O-sulfotransferase pipe (pip), partial

  • 中文名稱：
    黑腹果蠅pip重組蛋白
  • 貨號：
    CSB-YP801176DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅pip重組蛋白
  • 貨號：
    CSB-EP801176DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅pip重組蛋白
  • 貨號：
    CSB-EP801176DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅pip重組蛋白
  • 貨號：
    CSB-BP801176DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅pip重組蛋白
  • 貨號：
    CSB-MP801176DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    pip
  • Uniprot No.：
  • 別名：
    pip; CG9614; Heparan sulfate 2-O-sulfotransferase pipe; EC 2.8.2.-
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Sulfotransferase involved in dorsoventral axis patterning in early embryos. Required for the specific ventral activation of a series of proteases acting in the perivitelline space between the embryonic membrane and the eggshell. Probably acts by mediating the sulfation of some glycoprotein or glycosaminoglycan stably deposited in the egg, whose ventrally localized modification leads to spatially restricted activation of the protease cascade.
  • 基因功能參考文獻：
    1. Pipe modifies proteins that are incorporated in the ventral eggshell and activate Toll signaling which in turn initiates embryonic dorsoventral patterning. PMID: 22198544
    2. promotes 6-O- and/or N-sulfation of heparan sulfate but is not required for heparan sulfate 2-O-sulfation PMID: 18719407
    3. no requirement for nudel to be expressed in ventral, pipe-expressing follicle cells PMID: 18776742
    4. observation suggests a mechanism in which the sulfated target of Pipe promotes a productive interaction between Snake and Easter, perhaps by facilitating conformational changes in a complex containing the two proteins PMID: 20605458
    5. Snake activation is cascade-dependent but pipe-independent. Snake's activation of Easter is the first spatially regulated step in the dorsoventral protease cascade. PMID: 20638387
    6. Pipe induces ventral cell fates in the embryo via activation of the Spatzle/Toll pathway. PMID: 12050143
    7. Pipe acts as a sulfotransferase PMID: 16049108
    8. Sulfation of eggshell components by Pipe defines dorsal-ventral polarity in the Drosophila embryo. PMID: 19540119
    9. Data indicate that differences in functional specificity, and presumably enzymatic specificity, are associated with several of the Pipe isoforms. PMID: 19633171

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  • 亞細胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein. Note=Wbl is required for its processing into the Golgi.
  • 蛋白家族：
    Sulfotransferase 3 family
  • 組織特異性：
    Isoform 4 is ovary-specific. Isoform 4 and isoform 5 are specifically expressed in the ventral follicle cells of the egg chamber.
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG9614

    STRING: 7227.FBpp0074780

    UniGene: Dm.19224



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