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  4. Recombinant Drosophila melanogaster Guanine nucleotide-binding protein G (o) subunit alpha 47A

Recombinant Drosophila melanogaster Guanine nucleotide-binding protein G (o) subunit alpha 47A

  • 中文名稱：
    黑腹果蠅Galphao重組蛋白
  • 貨號：
    CSB-YP325422DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Galphao重組蛋白
  • 貨號：
    CSB-EP325422DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Galphao重組蛋白
  • 貨號：
    CSB-EP325422DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Galphao重組蛋白
  • 貨號：
    CSB-BP325422DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Galphao重組蛋白
  • 貨號：
    CSB-MP325422DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Galphao
  • Uniprot No.：
  • 別名：
    Galphao; G-oa47A; G-oalpha47A; CG2204G protein alpha o subunit; Guanine nucleotide-binding protein G(o) subunit alpha 47A
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-354
  • 氨基酸序列
    GCAQSAEER AAAARSRLIE RNLKEDGIQA AKDIKLLLLG AGESGKSTIV KQMKIIHESG FTAEDFKQYR PVVYSNTIQS LVAILRAMPT LSIQYSNNER ESDAKMVFDV CQRMHDTEPF SEELLAAMKR LWQDAGVQEC FSRSNEYQLN DSAKYFLDDL DRLGAKDYQP TEQDILRTRV KTTGIVEVHF SFKNLNFKLF DVGGQRSERK KWIHCFEDVT AIIFCVAMSE YDQVLHEDET TNRMQESLKL FDSICNNKWF TDTSIILFLN KKDLFEEKIR KSPLTICFPE YTGGQEYGEA AAYIQAQFEA KNKSTSKEIY CHMTCATDTN NIQFVFDAVT DVIIANNLRG CGLY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Plays a role in glial cell differentiation during embryogenesis; loco, Galphai and the G-protein coupled receptor, moody, are required in the surface glia to achieve effective insulation of the nerve cord.
  • 基因功能參考文獻：
    1. We have taken advantage of this approach to stabilize protein G alpha-go from Drosophila melanogaster. The designed SS-bridge increased by 4 degrees the melting temperature of one domain of protein G alpha-go . PMID: 26786871
    2. The heterotrimeric G protein Go functions as a transducer of Wingless-Frizzled 2 signaling in the synapse and Ankyrin 2 as a target of Go signaling required for neuromuscular junction formation. PMID: 25139856
    3. Galphao47A is required in bitter-sensitive taste neurons for L-canavanine sensitivity. PMID: 23671680
    4. Go is required for maximal physiological responses to multiple odorants in Drosophila, and olfactory receptor channel function and G-protein signaling are required for odor responsiveness. PMID: 19943954
    5. The roles for Galpha(o) subunit (Go) in mediating the two distinct pathways transduced by Fz receptors in Drosophila. PMID: 15652486

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  • 蛋白家族：
    G-alpha family, G(i/o/t/z) subfamily
  • 組織特異性：
    Expressed primarily in neuronal cell bodies in the brain, optic lobe, and thoracic and abdominal ganglia. Also expressed in antenna, oocytes and ovarian nurse cells.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG2204

    STRING: 7227.FBpp0087359

    UniGene: Dm.12982



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