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  4. Recombinant Drosophila melanogaster G protein-coupled receptor kinase 2 (Gprk2), partial

Recombinant Drosophila melanogaster G protein-coupled receptor kinase 2 (Gprk2), partial

  • 中文名稱：
    黑腹果蠅Gprk2重組蛋白
  • 貨號：
    CSB-YP341365DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Gprk2重組蛋白
  • 貨號：
    CSB-EP341365DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Gprk2重組蛋白
  • 貨號：
    CSB-EP341365DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Gprk2重組蛋白
  • 貨號：
    CSB-BP341365DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Gprk2重組蛋白
  • 貨號：
    CSB-MP341365DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Gprk2
  • Uniprot No.：
  • 別名：
    Gprk2; Gprk-2; CG17998; G protein-coupled receptor kinase 2; EC 2.7.11.16
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Specifically phosphorylates the activated forms of G protein-coupled receptors. Required during oogenesis and embryogenesis; component of a signaling pathway that functions during egg chamber maturation.
  • 基因功能參考文獻(xiàn)：
    1. pleckstrin homology domain of Gprk2 essential for enriching PI(4)P and facilitating Smo activation PMID: 26863604
    2. based on findings, propose that Gprk2 attenuates and tunes Fog-Cta signaling to prevent apical constriction in lateral mesodermal cells and to support appropriate apical constriction in ventral mesodermal cells PMID: 24026125
    3. Drosophila G-protein-coupled receptor kinase 2 regulates cAMP-dependent Hedgehog signaling. PMID: 22096079
    4. Oscillations in GPRK2 levels drive rhythms in gustatory physiology and behavior. PMID: 20153192
    5. Data show that G protein-coupled receptor 2 (Gprk2) expression is required in the germline for proper formation of the anterior egg structures and for early embryogenesis. PMID: 12535651
    6. Circadian-clock-dependent rhythms in GPRK2 abundance control the rhythmic accumulation of odorant receptors in olfactory sensory neuron dendrites. PMID: 18499458
    7. Smoothened (Smo) is regulated by distinct Ptc-dependent and Gprk2-dependent trafficking mechanisms in vivo. PMID: 19850026

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  • 亞細(xì)胞定位：
    Membrane. Note=Associated with nurse cell and oocyte plasma membranes during much of oogenesis.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, GPRK subfamily
  • 組織特異性：
    Expressed in all larval tissues and in adult ovaries. Larval CNS staining is localized to axons projecting to the optic lobes and the mushroom bodies, in the longitudinal connectives, and in cell bodies and nerves of the ring gland corpus allatum. Adult C
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG17998

    STRING: 7227.FBpp0085149

    UniGene: Dm.4430



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