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Recombinant Drosophila melanogaster Enhancer of yellow 2 transcription factor (e (y)2)

  • 中文名稱:
    黑腹果蠅e(y)2重組蛋白
  • 貨號:
    CSB-YP894756DLU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    黑腹果蠅e(y)2重組蛋白
  • 貨號:
    CSB-EP894756DLU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    黑腹果蠅e(y)2重組蛋白
  • 貨號:
    CSB-EP894756DLU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    黑腹果蠅e(y)2重組蛋白
  • 貨號:
    CSB-BP894756DLU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    黑腹果蠅e(y)2重組蛋白
  • 貨號:
    CSB-MP894756DLU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    e(y)2
  • Uniprot No.:
  • 別名:
    e(y)2; ENY2; CG15191Enhancer of yellow 2 transcription factor
  • 種屬:
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-101
  • 氨基酸序列
    MSTSGAVDQY TVLTGDRSKI KDLLCSRLTE CGWRDEVRLM CRNILMEKGT NNSFTVEQLI AEVTPKARTL VPDAVKKELL MKIRTILTEI EEEPDEPEDE S
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Involved in mRNA export coupled transcription activation by association with both the AMEX and the SAGA complexes. The SAGA complex is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates histone H2B. The SAGA complex is recruited to specific gene promoters by activators, where it is required for transcription. Required for nuclear receptor-mediated transactivation. Involved in transcription elongation by recruiting the THO complex onto nascent mRNA. The AMEX complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). AMEX participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Recruited to the su(Hw) insulators via its interaction with su(Hw) and participates in the barrier activity of such insulators. In contrast, it does not participate in the enhancer-blocking activity of the su(Hw) insulators.
  • 基因功能參考文獻:
    1. ENY2 protein forms a part of the THO complex of Drosophila melanogaster. PMID: 20714859
    2. 368-bp core of E(y)2 insulator is sufficient for blocking Polycomb response element-mediated silencing. PMID: 20082086
    3. The e(y)2 gene derived as a result of retroposition of the e(y)2b gene during Drosophila evolution. PMID: 16314324
    4. The e(y)2b gene is a paralogue of the e(y)2 gene. PMID: 16314324
    5. 3 additional subunits of TFTC/STAGA (ATXN7L3, USP22, & ENY2) help form the deubiquitination module. This module is an enhancer of position effect variegation in Drosophila. ATXN7L3 and USP22. PMID: 18206972
    6. E(y)2, the novel component of SAGA/TFTC complex in eucaryotes participates in export of mRNP from nucleus and couples transcription with nuclear pore PMID: 19425494
    7. E(y)2 and Xmas-2 together with SAGA/TFTC functioned in the anchoring of a subset of transcription sites to the NPCs to achieve efficient transcription and mRNA export. PMID: 19947544
    8. ENY2 in Drosophila is also stably associated with THO, the complex involved in mRNP biogenesis PMID: 20048002

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  • 亞細胞定位:
    Nucleus, nucleoplasm. Cytoplasm.
  • 蛋白家族:
    ENY2 family
  • 組織特異性:
    Ubiquitous.
  • 數據庫鏈接:

    KEGG: dme:Dmel_CG15191

    STRING: 7227.FBpp0073385

    UniGene: Dm.31083



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