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  4. Recombinant Drosophila melanogaster Enhancer of split m8 protein (E (spl))

Recombinant Drosophila melanogaster Enhancer of split m8 protein (E (spl))

  • 中文名稱：
    黑腹果蠅E(spl)重組蛋白
  • 貨號：
    CSB-YP320181DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅E(spl)重組蛋白
  • 貨號：
    CSB-EP320181DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅E(spl)重組蛋白
  • 貨號：
    CSB-EP320181DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅E(spl)重組蛋白
  • 貨號：
    CSB-BP320181DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅E(spl)重組蛋白
  • 貨號：
    CSB-MP320181DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    E(spl)
  • Uniprot No.：
  • 別名：
    E(spl)m8-HLH; E(spl); m8; CG8365Enhancer of split m8 protein; E(spl)m8
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-179
  • 氨基酸序列
    MEYTTKTQIY QKVKKPMLER QRRARMNKCL DNLKTLVAEL RGDDGILRMD KAEMLESAVI FMRQQKTPKK VAQEEQSLPL DSFKNGYMNA VNEVSRVMAS TPGMSVDLGK SVMTHLGRVY KNLQQFHEAQ SAADFIQNSM DCSSMDKAPL SPASSGYHSD CDSPAPSPQP MQQPLWRPW
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Participates in the control of cell fate choice by uncommitted neuroectodermal cells in the embryo. Transcriptional repressor. Binds DNA on N-box motifs: 5'-CACNAG-3'. Part of the Notch signaling pathway.
  • 基因功能參考文獻：
    1. Studies identify PP2A-Wdb as a participant in Notch signaling, and suggest that M8 activity is controlled by phosphorylation and dephosphorylation. PMID: 25006677
    2. Based on these data, we propose a model in which Hes genes are responsible for co-ordinating the Notch response of a wide spectrum of other targets, explaining the critical functions these key regulators play in many developmental and disease contexts. PMID: 23300480
    3. The phosphorylation domain of M8 is implicated in autoinhibition, and indicate that binding of the co-repressor Groucho (Gro) is context-dependent. PMID: 21789514
    4. Drosophila STUbL protein Degringolade limits HES functions during embryogenesis. PMID: 21486924
    5. Studies provide evidence that autoinhibition by the CtD regulates M8 activity in a phosphorylation-dependent manner. PMID: 20014344
    6. Polymorphisms in the enhancer of split gene complex affect bristle number variation in a large wild-caught cohort of Drosophila melanogaster. PMID: 16143618

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  • 亞細胞定位：
    Nucleus.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG8365

    STRING: 7227.FBpp0084335

    UniGene: Dm.27416



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