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  4. Recombinant Drosophila melanogaster Endoribonuclease Dcr-1 (Dcr-1), partial

Recombinant Drosophila melanogaster Endoribonuclease Dcr-1 (Dcr-1), partial

  • 中文名稱：
    Recombinant Drosophila melanogaster Endoribonuclease Dcr-1(Dcr-1) ,partial
  • 貨號：
    CSB-YP893259DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Endoribonuclease Dcr-1(Dcr-1) ,partial
  • 貨號：
    CSB-EP893259DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Endoribonuclease Dcr-1(Dcr-1) ,partial
  • 貨號：
    CSB-EP893259DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Endoribonuclease Dcr-1(Dcr-1) ,partial
  • 貨號：
    CSB-BP893259DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Endoribonuclease Dcr-1(Dcr-1) ,partial
  • 貨號：
    CSB-MP893259DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Dcr-1
  • Uniprot No.：
  • 別名：
    Dcr-1; CG4792Endoribonuclease Dcr-1; Protein dicer-1; EC 3.1.26.-
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Essential for RNA interference (RNAi); double-stranded RNA (dsRNA) induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger. May carry out the initiation step of RNAi by cleaving dsRNA to produce 22 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs. During embryogenesis, involved in germline fate determination.
  • 基因功能參考文獻：
    1. [review] Dicer-1 lacks a functional DExD/H helicase domain and is responsible for processing hairpin-structured pre-microRNAs. PMID: 24556400
    2. Dicer-1 is required for siRNA-directed mRNA cleavage. Dicer-1 facilitates distinct steps in the assembly of siRISC, and is essential for miRISC-directed translation repression. PMID: 15066283
    3. Dcr-1 is critical for assembly of microRNA as shown in dcr-1 knockout egg extract PMID: 17928574
    4. These results reveal a novel interaction between Flock house virus B2 protein and Dicer that leads to suppression of siRNA biogenesis. PMID: 19193719
  • 蛋白家族：
    Helicase family, Dicer subfamily
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG4792

    STRING: 7227.FBpp0083717

    UniGene: Dm.1283



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