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  4. Recombinant Drosophila melanogaster Endophilin-A (endoA)

Recombinant Drosophila melanogaster Endophilin-A (endoA)

  • 中文名稱：
    黑腹果蠅EndoA重組蛋白
  • 貨號：
    CSB-YP844691DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅EndoA重組蛋白
  • 貨號：
    CSB-EP844691DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅EndoA重組蛋白
  • 貨號：
    CSB-EP844691DLU-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅EndoA重組蛋白
  • 貨號：
    CSB-BP844691DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅EndoA重組蛋白
  • 貨號：
    CSB-MP844691DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    EndoA
  • Uniprot No.：
  • 別名：
    EndoA; endo; CG14296; Endophilin-A; SH3 domain-containing GRB2-like protein
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-369
  • 氨基酸序列
    MAFAGLKKQI NKANQYMTEK MGGAEGTKLD MDFMEMERKT DVTVELVEEL QLKTKEFLQP NPTARAKMAA VKGISKLSGQ AKSNTYPQPE GLLAECMLTY GKKLGEDNSV FAQALVEFGE ALKQMADVKY SLDDNIKQNF LEPLHHMQTK DLKEVMHHRK KLQGRRLDFD CKRRRQAKDD EIRGAEDKFG ESLQLAQVGM FNLLENDTEH VSQLVTFAEA LYDFHSQCAD VLRGLQETLQ EKRSEAESRP RNEFVPKTLL DLNLDGGGGG LNEDGTPSHI SSSASPLPSP MRSPAKSMAV TPQRQQQPCC QALYDFEPEN PGELAFKEND IITLLNRVDD NWFEGAVNGR TGYFPQSYVQ VQVPLPNGN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required presynaptically at the neuromuscular junction. Implicated in synaptic vesicle endocytosis.
  • 基因功能參考文獻：
    1. EndophilinA-induced macroautophagy is activated when the kinase LRRK2 phosphorylates the EndophilinA-BAR domain and is blocked in animals where EndophilinA cannot be phosphorylated. Functionally, deregulation of the EndophilinA phosphorylation state accelerates activity-induced neurodegeneration. PMID: 27720484
    2. analysis of endophilin-A N-BAR domain mutations performed in living flies PMID: 20209138
    3. endophilin A is essential for synaptic vesicle endocytosis, being required from the onset of budding until fission. PMID: 11927550
    4. Endophilin is required presynaptically at the neuromuscular junction, and absence of Endophilin dramatically impairs endocytosis in vivo PMID: 11955450
    5. At fly photoreceptor synapses Endophilin is localized to synaptic vesicles at sites of endocytosis that are glial invaginations called capitate projections; when the photoreceptor synapses lack Endophilin they are impaired in neurotransmitter release PMID: 14627659

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  • 亞細胞定位：
    Cytoplasm. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Endophilin family
  • 組織特異性：
    Expression is abundant in central nervous system (brain lobes and the ventral nerve cord) starting at embryonic stage 13 through to third instar larvae. Also expressed in photoreceptors in the eye imaginal disks and the Bolwig organs of the peripheral ner
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG14296

    STRING: 7227.FBpp0083112

    UniGene: Dm.3503



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