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Recombinant Drosophila melanogaster Clathrin heavy chain (Chc), partial

  • 中文名稱:
    Recombinant Drosophila melanogaster Clathrin heavy chain(Chc),partial
  • 貨號:
    CSB-EP333472DLU
  • 規格:
    ¥1836
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性:
    Not Test
  • 基因名:
    Chc
  • Uniprot No.:
  • 種屬:
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度:
    Partial
  • 來源:
    E.coli
  • 分子量:
    33
  • 表達區域:
    1-260aa
  • 氨基酸序列
    MTQPLPIRFQEHLQLTNVGINANSFSFSTLTMESDKFICVREKVNDTAQVVIIDMNDATNPTRRPISADSAIMNPASKVIALKAQKTLQIFNIEMKSKMKAHTMNEDVVFWKWISLNTLALVTETSVFHWSMEGDSMPQKMFDRHSSLNGCQIINYRCNASQQWLLLVGISALPSRVAGAMQLYSVERKVSQAIEGHAASFATFKIDANKEPTTLFCFAVRTATGGKLHIIEVGAPPNGNQPFAKKAVDVFFPPEAQNDF
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
  • 基因功能參考文獻:
    1. Bic-D is also required to transport Chc mRNA into the oocyte and for transport and proper localization of Chc protein to the oocyte cortex. PMID: 24718986
    2. Results report a Drosophila disabled (DAB)mutant revealing a novel role for DAB proteins in chemical synaptic transmission, and support a role for clathrin-mediated endocytosis in rapid clearance of neurotransmitter release sites. PMID: 21606364
    3. Bicaudal-D binds clathrin heavy chain to promote its transport and augments synaptic vesicle recycling. PMID: 20111007
    4. chc mRNA and protein are highly expressed from early stages of embryogenesis onwards, consistent with genetic studies predicting a maternal contribution of the gene function PMID: 19577664
    5. Chc is critical for synaptic vesicle recycling and in the absence of the protein, bulk retrieval mediates massive synaptic membrane internalization. PMID: 18762582

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  • 亞細胞定位:
    Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic face of coated pits and vesicles.
  • 蛋白家族:
    Clathrin heavy chain family
  • 數據庫鏈接:

    KEGG: dme:Dmel_CG9012

    STRING: 7227.FBpp0073966

    UniGene: Dm.3174



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