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  4. Recombinant Drosophila melanogaster Centromere/kinetochore protein zw10 (mit (1)15), partial

Recombinant Drosophila melanogaster Centromere/kinetochore protein zw10 (mit (1)15), partial

  • 中文名稱：
    黑腹果蠅Zw10重組蛋白
  • 貨號：
    CSB-YP893371DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Zw10重組蛋白
  • 貨號：
    CSB-EP893371DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Zw10重組蛋白
  • 貨號：
    CSB-EP893371DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Zw10重組蛋白
  • 貨號：
    CSB-BP893371DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Zw10重組蛋白
  • 貨號：
    CSB-MP893371DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Zw10
  • Uniprot No.：
  • 別名：
    Zw10; mit(1)15; CG9900Centromere/kinetochore protein zw10; Mitotic 15 protein
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin, Mad2 complexes and spindly/CG15415 onto kinetochores. During cytokinesis in male meiotic cells it is required for completion of cleavage furrow ingression, possibly in conjunction with Rint1. Required for maintenance of Golgi stack number and morphology, and acroblast assembly. Its function related to the spindle assembly machinery is proposed to depend on its association in the RZZ complex. Failure to assemble the complex due to the absence of any one of its components, results in the incorrect redistribution of the remaining components to diverse membrane compartments.
  • 基因功能參考文獻：
    1. Rod/Zw10 contributes to checkpoint activation by promoting Mad2 recruitment and to checkpoint inactivation by recruiting dynein/dynactin that subsequently removes Mad2 from attached kinetochores. PMID: 15886105
    2. ZW10 has roles in mitotic checkpoint control, dynein targeting and membrane trafficking with dynein [review] PMID: 17102640
  • 亞細胞定位：
    Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Golgi apparatus. Golgi apparatus, Golgi stack.
  • 蛋白家族：
    ZW10 family
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG9900

    STRING: 7227.FBpp0070425

    UniGene: Dm.1760



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