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  4. Recombinant Drosophila melanogaster Adenosine monophosphate-protein transferase FICD homolog (CG9523), partial

Recombinant Drosophila melanogaster Adenosine monophosphate-protein transferase FICD homolog (CG9523), partial

  • 中文名稱：
    黑腹果蠅Fic重組蛋白
  • 貨號：
    CSB-YP844681DLU1
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Fic重組蛋白
  • 貨號：
    CSB-EP844681DLU1
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Fic重組蛋白
  • 貨號：
    CSB-EP844681DLU1-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Fic重組蛋白
  • 貨號：
    CSB-BP844681DLU1
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Fic重組蛋白
  • 貨號：
    CSB-MP844681DLU1
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Fic
  • Uniprot No.：
  • 別名：
    Fic; CG9523; Protein adenylyltransferase Fic; dFic; De-AMPylase Fic
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-247 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of Hsc70-3/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of Hsc70-3/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from Hsc70-3/BiP at 'Thr-518', leading to restore HSPA5/BiP activity.
  • 基因功能參考文獻：
    1. Data (including data from studies using transgenic animals) suggest that dual enzymatic activity of Fic is, in part, regulated by Fic dimerization, as loss of this dimerization increases AMPylation and reduces deAMPylation of BiP/Grp78. (Fic = Fic domain-containing protein; BiP/Grp78 = molecular chaperone BiP) PMID: 29089387
    2. The inactive conformation of BiP is the preferred substrate for dFic. PMID: 25395623
    3. Our data are consistent with the idea that Fic's enzymatic activity is involved in the regulation of the distribution of the visual neurotransmitter histamine PMID: 22544313
  • 亞細胞定位：
    Membrane; Single-pass membrane protein.
  • 蛋白家族：
    Fic family
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG9523

    STRING: 7227.FBpp0078887

    UniGene: Dm.11352



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