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  4. Recombinant Drosophila melanogaster 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (norpA), partial

Recombinant Drosophila melanogaster 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (norpA), partial

  • 中文名稱：
    黑腹果蠅norpA重組蛋白
  • 貨號：
    CSB-YP319059DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅norpA重組蛋白
  • 貨號：
    CSB-EP319059DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅norpA重組蛋白
  • 貨號：
    CSB-EP319059DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅norpA重組蛋白
  • 貨號：
    CSB-BP319059DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅norpA重組蛋白
  • 貨號：
    CSB-MP319059DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    norpA
  • Uniprot No.：
  • 別名：
    norpA; PLC-beta; CG3620; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase; EC 3.1.4.11; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta; No receptor potential A protein; Phosphoinositide phospholipase C; Phosphoinositide phospholipase C-beta
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential component of the phototransduction pathway. Essential downstream component of a hh-signaling pathway which regulates the Duox-dependent gut immune response to bacterial uracil; required for the activation of Cad99C and consequently Cad99C-dependent endosome formation, which is essential for the Duox-dependent production of reactive oxygen species (ROS) in response to intestinal bacterial infection.
  • 基因功能參考文獻：
    1. This study demonstrated that NorpA and eya2 mutants with disrupted vision showed normal mating effects on sleep. PMID: 28851647
    2. These results provide evidence for the existence of a unique dPIP5K dependent pool of PIP2 required for normal Drosophila phototransduction. PMID: 25633995
    3. The positive feedback between PLCbeta and IP3R found here may represent a common feature of the inositol-lipid signaling PMID: 25673847
    4. Taken together, these findings reveal that PLC function in Drosophila sweet-sensing neurons is a conserved molecular signaling pathway that confers attraction to fatty acids PMID: 24068941
    5. This review prestnted the evidence the norpA mutation have significantly contributed to understanding the molecular and biochemical basis of phototransduction in Drosophila. PMID: 22283804
    6. Light reception mediated by Rh5 and Rh6 must utilize either a different (nonretinal) phospholipase C-beta enzyme or alternative signaling mechanisms, at least in terms of clock-relevant photoreception PMID: 22306971
    7. loss of the norpa gene product induces massive endocytosis of rhodopsin and arrestin in photoreceptors PMID: 11853766
    8. Mutants of norpA, encoding phospholipase C beta (PLC beta), attenuate response to diuretic peptides of Drosophila melanogaster renal (Malpighian) tubules PMID: 12547945
    9. norpA(P41) is directly involved in thermosensitivity PMID: 14766972
    10. Of 47 norpA mutants screened for PLC protein content, all but one displayed drastically decreased amounts of protein suggesting that almost any mutational alteration has a deleterious effect on the integrity of the protein PMID: 15140198
    11. Analysis of norpA inaE double mutants and severe inaE mutants defective in photoreceptor respponses to light shows that normal diacylglycerol lipase activity is required for the generation of physiologically meaningful photoreceptor responses. PMID: 18579079
    12. the Galphaq-PLCbeta-Ca(2+)-DUOX-ROS signaling pathway acts as a bona fide first line of defense that enables gut epithelia to dynamically control yeast during the Drosophila life cycle PMID: 19289084

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  • 組織特異性：
    Abundantly expressed in the adult retina.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG3620

    STRING: 7227.FBpp0070618

    UniGene: Dm.7394



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