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  4. Recombinant Dog Gastric triacylglycerol lipase (LIPF)

Recombinant Dog Gastric triacylglycerol lipase (LIPF)

  • 中文名稱：
    熟悉的犬LIPF重組蛋白
  • 貨號：
    CSB-YP012976DO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    熟悉的犬LIPF重組蛋白
  • 貨號：
    CSB-EP012976DO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    熟悉的犬LIPF重組蛋白
  • 貨號：
    CSB-EP012976DO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    熟悉的犬LIPF重組蛋白
  • 貨號：
    CSB-BP012976DO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    LIPF
  • Uniprot No.：
  • 別名：
    LIPFGastric triacylglycerol lipase; GL; Gastric lipase; EC 3.1.1.3
  • 種屬：
    Canis familiaris (Dog) (Canis lupus familiaris)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    20-398
  • 氨基酸序列
    L FGKLHPTNPE VTMNISQMIT YWGYPAEEYE VVTEDGYILG IDRIPYGRKN SENIGRRPVA FLQHGLLASA TNWISNLPNN SLAFILADAG YDVWLGNSRG NTWARRNLYY SPDSVEFWAF SFDEMAKYDL PATIDFILKK TGQDKLHYVG HSQGTTIGFI AFSTNPKLAK RIKTFYALAP VATVKYTETL LNKLMLVPSF LFKLIFGNKI FYPHHFFDQF LATEVCSRET VDLLCSNALF IICGFDTMNL NMSRLDVYLS HNPAGTSVQN VLHWSQAVKS GKFQAFDWGS PVQNMMHYHQ SMPPYYNLTD MHVPIAVWNG GNDLLADPHD VDLLLSKLPN LIYHRKIPPY NHLDFIWAMD APQAVYNEIV SMMGTDNK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the hydrolysis of triacylglycerols to yield free fatty acids, diacylglycerol, monoacylglycerol, and glycerol. Shows a preferential hydrolysis at the sn-3 position of triacylglycerol.
  • 基因功能參考文獻：
    1. We used b-lactoglobulin and thermosensitive methylated nanocrystalline cellulose (metNCC) as model biopolymers to investigate the role of interfacial fluid dynamics and morphology for interfacial displacement processes by rDGL and polysorbate 20 (P20) under gastric conditions. PMID: 27635994
    2. insights into the domain movements PMID: 20965171
    3. results show that gastric lipase also acts in solution on vinyl butyrate, with an optimum activity above pH 7, which suggests that gastric lipase is able to hydrolyze ester bonds via the classical mechanism of serine hydrolases PMID: 16411775
    4. Investigation of the influence of feeding and fasting on the daily rhythm of serum lipase activity in dogs. PMID: 18987232
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 組織特異性：
    Expressed in the mucous pit cells of gastric glands (at protein level). Expressed in the fundic and pyloric mucosa.
  • 數據庫鏈接：


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