Recombinant Dictyostelium discoideum Suppressor of Mek1 (smkA), partial
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中文名稱:盤基網柄菌smkA重組蛋白
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貨號:CSB-EP706734DKK-B
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說明書:
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規格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
產品詳情
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純度:>85% (SDS-PAGE)
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基因名:smkA
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Uniprot No.:
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別名:smkA; DDB_G0289067; Suppressor of Mek1; SMEK
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種屬:Dictyostelium discoideum (Slime mold)
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蛋白長度:Partial
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蛋白標簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
靶點詳情
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功能:Suppresses MEK1 null cell polarity, chemotaxis, and gene expression defects. Required for proper cytokinesis during vegetative growth, timely exit from the mound stage during development, and myosin II assembly. May be a regulatory subunit of serine/threonine-protein phosphatase 4 (PP4) and may control localization of PP4 to the nucleus. Involved in the regulation of some ppp4c functions, such as developmental progression, chemotaxis, expression of stress response genes and cell movement.
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基因功能參考文獻:
- smkA (Supressor of mekA) regulates chemotaxis and the expression of genes specifically involved in cell stress responses and cell movement. PMID: 17353263
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亞細胞定位:Cytoplasm, cell cortex. Nucleus. Note=Localizes to the cell cortex in vegetative cells but translocates to the nucleus during starvation and development (PubMed:16107728).
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蛋白家族:SMEK family
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數據庫鏈接:
KEGG: ddi:DDB_G0289067
STRING: 44689.DDB0232046
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