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  4. Recombinant Dictyostelium discoideum Gem-associated protein 2 (gemin2)

Recombinant Dictyostelium discoideum Gem-associated protein 2 (gemin2)

  • 中文名稱：
    盤基網柄菌gemin2重組蛋白
  • 貨號：
    CSB-YP687629DKK
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    盤基網柄菌gemin2重組蛋白
  • 貨號：
    CSB-EP687629DKK
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    盤基網柄菌gemin2重組蛋白
  • 貨號：
    CSB-EP687629DKK-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    盤基網柄菌gemin2重組蛋白
  • 貨號：
    CSB-BP687629DKK
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    盤基網柄菌gemin2重組蛋白
  • 貨號：
    CSB-MP687629DKK
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    gemin2
  • Uniprot No.：
  • 別名：
    gemin2; DDB_G0287253Gem-associated protein 2; Gemin-2; Component of gems protein 2
  • 種屬：
    Dictyostelium discoideum (Slime mold)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-331
  • 氨基酸序列
    MDEFQSKAFE VGEEIEPDDN EPLTGEEYLQ RVKWHSNRCP SVVVADIDYS KIKVTIPSNS YFTLPPSITK CKKELLPTPT WEKEFLNDFS EFRQKLQYIK SNRPSNNNNN NNNNNNNNNN NNNNNNLIPQ LPHINDKRYW YIFCFGSNGN NNNNNNDIKM KDFNDNQEDD DDDENNEDYE YNENKEEEEE EEEEEEEEEE VEEEEEEEEE EEEVVDYSTK KPTLGNKPTM DILCRLDHVL TVALVNYHIE WLEKREFTQE RSYWLYMLLS LLEKPIDPDT CSNLRSCIRR LSVFRSKITN LNDPNLPSIN ILFTIIAKYF DQLEPSDILY L
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound.; May play an essential role in spliceosomal snRNP assembly in the cytoplasm and may be required for pre-mRNA splicing in the nucleus.
  • 亞細胞定位：
    Nucleus, gem. Cytoplasm.
  • 蛋白家族：
    Gemin-2 family
  • 數據庫鏈接：


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