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  4. Recombinant Degenerin mec-4 (mec-4), partial

Recombinant Degenerin mec-4 (mec-4), partial

  • 中文名稱：
    秀麗隱桿線蟲mec-4重組蛋白
  • 貨號：
    CSB-YP326156CXY
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mec-4重組蛋白
  • 貨號：
    CSB-EP326156CXY
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mec-4重組蛋白
  • 貨號：
    CSB-EP326156CXY-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mec-4重組蛋白
  • 貨號：
    CSB-BP326156CXY
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mec-4重組蛋白
  • 貨號：
    CSB-MP326156CXY
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mec-4
  • Uniprot No.：
  • 別名：
    mec-4; mec-13; T01C8.7Degenerin mec-4; Mechanosensory abnormality protein 4
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Subunit of an amiloride-sensitive cation channel (degenerin channel complex) permeable for sodium, potassium, lithium and N-methylglucamine, and required for mechanosensory transduction (touch sensitivity). Negatively regulates the turning step of male mating behavior.
  • 基因功能參考文獻：
    1. Ubiquitination of MEC-4 reduces the amount of MEC-4 protein in the processes of ALM neurons and, consequently, the mechanoreceptor current. PMID: 25653375
    2. bz2 mutation encodes substitution A745T, which is situated in the intracellular C-terminal domain of MEC-4. We show that this substitution renders both MEC-4 and MEC-4(d) activity strongly temperature sensitive PMID: 16239217
    3. The d position is part of a key region in the channel functionally and structurally, possibly representing the beginning of a pore-forming domain. PMID: 17261841
    4. MEC-4/MEC-10 DEG/ENaC or its partners MEC-6, MEC-7, and MEC-9 blocked DAF-16::GFP nuclear accumulation induced by hypergravity but did not affect DAF-16 response to other stresses PMID: 17720915
    5. MEC-4 is distributed essentially evenly with MEC-2 around the circumference of touch receptor neurites that serve as gating tethers critical for channel activation by mechano-electrical transduction. PMID: 18094248

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  • 亞細胞定位：
    Membrane; Multi-pass membrane protein. Cell projection, axon.
  • 蛋白家族：
    Amiloride-sensitive sodium channel (TC 1.A.6) family
  • 組織特異性：
    Expressed in AVM, ALM, PVM and PLM touch neurons.
  • 數據庫鏈接：

    KEGG: cel:CELE_T01C8.7

    STRING: 6239.T01C8.7.2

    UniGene: Cel.634



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