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  4. Recombinant Danio rerio Piwi-like protein 2 (piwil2), partial

Recombinant Danio rerio Piwi-like protein 2 (piwil2), partial

  • 中文名稱：
    斑馬魚piwil2重組蛋白
  • 貨號：
    CSB-YP018046DIL
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    斑馬魚piwil2重組蛋白
  • 貨號：
    CSB-EP018046DIL
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    斑馬魚piwil2重組蛋白
  • 貨號：
    CSB-EP018046DIL-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    斑馬魚piwil2重組蛋白
  • 貨號：
    CSB-BP018046DIL
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    斑馬魚piwil2重組蛋白
  • 貨號：
    CSB-MP018046DIL
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    piwil2
  • Uniprot No.：
  • 別名：
    piwil2; zili; si:dkey-88f5.1; Piwi-like protein 2; EC 3.1.26.-
  • 種屬：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in germ cell differentiation and meiosis, independently of the function in transposable elements repression. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' piwil4. Piwil2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded. Required for piwil4/miwi2 nuclear localization and association with secondary piRNAs antisense. Represses circadian rhythms by promoting the stability and activity of core clock components ARNTL/BMAL1 and CLOCK.
  • 基因功能參考文獻：
    1. zili might have some effect on the formation of the ectoderm, mesoderm and endoderm during early embryogenesis PMID: 21265086
    2. Tdrd1 binds both zebrafish Piwi proteins, Ziwi and Zili, and reveals sequence specificity in the interaction between Tdrd1 tudor domains and symmetrically dimethylated arginines (sDMAs) in Zili. PMID: 21743441
    3. These results indicate that zili regulates dorsal-ventral patterning by antagonizing Bmp signaling during early embryogenesis in zebrafish. PMID: 21627449
  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Argonaute family, Piwi subfamily
  • 組織特異性：
    Detected in primordial germ cells (PGCs) from 3 dpf. In adult, it is found in both the female and male gonad. In the ovary, it is present in all stages of germ cell differentiation. In testis, it is present in mitotic and meiotic germ cells. No protein ha
  • 數據庫鏈接：


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