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  4. Recombinant Danio rerio Piwi-like protein 1 (piwil1), partial

Recombinant Danio rerio Piwi-like protein 1 (piwil1), partial

  • 中文名稱：
    斑馬魚piwil1重組蛋白
  • 貨號：
    CSB-YP844967DIL
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    斑馬魚piwil1重組蛋白
  • 貨號：
    CSB-EP844967DIL
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    斑馬魚piwil1重組蛋白
  • 貨號：
    CSB-EP844967DIL-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    斑馬魚piwil1重組蛋白
  • 貨號：
    CSB-BP844967DIL
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    斑馬魚piwil1重組蛋白
  • 貨號：
    CSB-MP844967DIL
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    piwil1; ziwi; Piwi-like protein 1; EC 3.1.26.-
  • 種屬：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays a central role during gametogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Has a strong preference for piRNAs with a uridine nucleotide at their 5'-end (g1U preference, also named 1U-bias) and binds piRNAs in an opposite direction compared to piwil2/zili. Participates in a piRNA amplification loop with piwil2/zili. Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs.
  • 基因功能參考文獻(xiàn)：
    1. the zebrafish homologue of the Drosophila piwi: co-localization with vasa at the embryonic genital ridge and gonad-specific expression in the adults (ziwi protein). PMID: 12617811
    2. Tdrd1 acts as a molecular scaffold for Ziwi. PMID: 21743441
    3. Promoter activity of a 4.8-kb sequence isolated from the 5' end of the zebrafish germ cell-specific ziwi gene is sufficient to drive gene expression in all germ cells with stage-specific differences in expression levels. PMID: 20737503
    4. study demonstrated that zebrafish Piwi, Ziwi is expressed in gonad of both sexes and is a component of a structure called nuage; loss of Ziwi function results in a progressive loss of germ cells due to apoptosis during larval development PMID: 17418787
    5. Ziwi and Zili bind piRNAs of opposite polarity PMID: 18833190

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Argonaute family, Piwi subfamily
  • 組織特異性：
    Expressed exclusively in the adult gonads; expression in the ovary weaker than in the testis (at protein level). During neurogenesis and organogenesis, expression is detected in CNS (midbrain and eye) and fin buds. Starting from 24 hours post-fertilizatio
  • 數(shù)據(jù)庫鏈接：


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